EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.215 | gene ga2dh | Gluconobacter japonicus |
1.1.99.4 | expressed in Gluconobacter japonicas strain NBRC3271 | Gluconobacter oxydans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.215 | additional information | a mutant strain of Gluconobacter japonicus NBRC3271 is engineered to produce 2-dehydro-D-gluconate efficiently from a mixture of D-glucose and D-gluconate | Gluconobacter japonicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.215 | membrane | - |
Gluconobacter japonicus | 16020 | - |
1.1.99.4 | membrane | - |
Gluconobacter oxydans | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.215 | D-gluconate + NADP+ | Gluconobacter japonicus | - |
2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
1.1.1.215 | D-gluconate + NADP+ | Gluconobacter japonicus NBRC3271 | - |
2-dehydro-D-gluconate + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.215 | Gluconobacter japonicus | - |
- |
- |
1.1.1.215 | Gluconobacter japonicus NBRC3271 | - |
- |
- |
1.1.99.4 | Gluconobacter oxydans | A0A0B6VQJ4 and A0A0B6VN56 and A0A0B6VKP6 | cytochrome c subunit and large subunit and small subunit | - |
1.1.99.4 | Gluconobacter oxydans NBRC3293 | A0A0B6VQJ4 and A0A0B6VN56 and A0A0B6VKP6 | cytochrome c subunit and large subunit and small subunit | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.215 | D-gluconate + NADP+ | - |
Gluconobacter japonicus | 2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
1.1.1.215 | D-gluconate + NADP+ | - |
Gluconobacter japonicus NBRC3271 | 2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
1.1.99.4 | 2-dehydro-D-gluconate + ferricyanide | - |
Gluconobacter oxydans | 2,5-didehydro-D-gluconate + ferrocyanide | - |
? | |
1.1.99.4 | 2-dehydro-D-gluconate + ferricyanide | - |
Gluconobacter oxydans NBRC3293 | 2,5-didehydro-D-gluconate + ferrocyanide | - |
? | |
1.1.99.4 | additional information | the enzyme is completely inert toward 5-oxoglutarate, D-gluconate, D-glucose, D-sorbitol, L-lactate, L-malate, and D-fructose | Gluconobacter oxydans | ? | - |
? | |
1.1.99.4 | additional information | the enzyme is completely inert toward 5-oxoglutarate, D-gluconate, D-glucose, D-sorbitol, L-lactate, L-malate, and D-fructose | Gluconobacter oxydans NBRC3293 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.4 | trimer | a small subunit, a large subunit, and a cytochrome c subunit | Gluconobacter oxydans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.215 | 2-ketogluconate dehydrogenase | - |
Gluconobacter japonicus |
1.1.1.215 | GADH | - |
Gluconobacter japonicus |
1.1.99.4 | 2-keto-gluconate dehydrogenase | - |
Gluconobacter oxydans |
1.1.99.4 | 2KGDH | - |
Gluconobacter oxydans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.215 | NADP+ | - |
Gluconobacter japonicus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.215 | physiological function | Gluconobacter oxydans NBRC3293 produces 2,5-dioxo-D-gluconate from D-glucose via D-gluconate and 2-keto-D-gluconate, with accumulation of the product in the culture medium, the efficiency of 2,5-diketo-D-gluconate production is unsatisfactory because there is a large amount of residual D-gluconate at the end of the biotransformation process. Heterologous overexpression of the kgdSLC genes in a mutant strain of Gluconobacter japonicus NBRC3271 engineered to produce 2-dehydro-D-gluconate efficiently from a mixture of D-glucose and D-gluconate, results in a mutant strain that consumes almost all of the starting materials (D-glucose and D-gluconate) to produce 2,5-dioxo-D-gluconate quantitatively as a seemingly unique metabolite | Gluconobacter japonicus |