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Literature summary extracted from

  • Huecherig, S.; Petersen, M.
    RNAi suppression and overexpression studies of hydroxyphenylpyruvate reductase (HPPR) and rosmarinic acid synthase (RAS) genes related to rosmarinic acid biosynthesis in hairy root cultures of Coleus blumei (2013), Plant Cell Tissue Organ Cult., 113, 375-385.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.237 gene CbHPPR, DNA and amino acid sequence determination and analysis, hairy root lines of Coleus blumei carrying HPPR RNAi suppression and overexpression constructs are established using Agrobacterium rhizogenes strain LBA15834-mediated transformation, HPPR is overexpressed under control of the constitutive CaMV 35S-promoter Coleus scutellarioides

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.237 additional information hairy root lines of Coleus blumei carrying HPPR or rosmarinic acid synthase (RAS) RNAi suppression and overexpression constructs are established using Agrobacterium rhizogenes-mediated transformation. Hairy root lines showing undetectable HPPR or RAS mRNA levels displayed strongly reduced rosmarinic acid contents with reduction of up to 92% compared to control hairy root lines. An increase of rosmarinic acid levels up to 176% occurs in overexpression hairy root lines with equally increased HPPR transcript levels while RAS overexpression results in co-suppression effects with reduced rosmarinic acid levels Coleus scutellarioides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.237 34000
-
 2 * 34000, SDS-PAGE Coleus scutellarioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.237 3-(4-hydroxyphenyl)pyruvate + NADH + H+ Coleus scutellarioides
-
 3-(4-hydroxyphenyl)lactate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.237 Coleus scutellarioides Q65CJ7 i.e. Plectranthus scutellarioides
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.237 hairy root
-
 Coleus scutellarioides
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.237 3-(4-hydroxyphenyl)pyruvate + NADH + H+
-
 Coleus scutellarioides 3-(4-hydroxyphenyl)lactate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.237 homodimer 2 * 34000, SDS-PAGE Coleus scutellarioides

Synonyms

EC Number Synonyms Comment Organism
1.1.1.237 HPPR
-
 Coleus scutellarioides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.237 NADH
-
 Coleus scutellarioides

General Information

EC Number General Information Comment Organism
1.1.1.237 evolution HPPR belongs to the family of D-isomer-specific 2-hydroxyacid dehydrogenases Coleus scutellarioides
1.1.1.237 metabolism the enzyme is involved in biosynthesis of rosmarinic acid via the tyrosine-derived pathway, overview. Activity of HPPR seems to be a arte-limiting point in rosmarinic acid biosynthesis Coleus scutellarioides