EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.61 | Polyethylene glycol | enhances activity | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.61 | 1,10-phenanthroline | - |
Pseudomonas putida | |
1.14.13.61 | 4,5-dihydroxy-1,3-benzene disulfonic acid | - |
Pseudomonas putida | |
1.14.13.61 | EDTA | - |
Pseudomonas putida | |
1.14.13.61 | iodoacetate | - |
Pseudomonas putida | |
1.14.13.61 | NEM | - |
Pseudomonas putida | |
1.14.13.61 | p-hydroxymercuribenzoate | - |
Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.61 | Fe2+ | enhances activity | Pseudomonas putida | |
1.14.13.61 | Iron | the reductase component contains 1 plant-type ferredoxin [2Fe-2S] cluster, the oxygenase component contains 6 Rieske-type [2Fe-2S]clusters and additional iron | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.61 | 37000 | - |
the reductase component is a monomer, 1 * 37000, SDS-PAGE. The oxygenase component is a hexamer, 6 * 55000, SDS-PAGE | Pseudomonas putida |
1.14.13.61 | 39000 | - |
reductase component, gel filtration | Pseudomonas putida |
1.14.13.61 | 55000 | - |
the reductase component is a monomer, 1 * 37000, SDS-PAGE. The oxygenase component is a hexamer, 6 * 55000, SDS-PAGE | Pseudomonas putida |
1.14.13.61 | 330000 | - |
oxygenase component, gel filtration | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.61 | 2-Oxo-1,2-dihydroquinoline + NADH + O2 | Pseudomonas putida | second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline | ? | - |
? | |
1.14.13.61 | 2-Oxo-1,2-dihydroquinoline + NADH + O2 | Pseudomonas putida 86 | second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.61 | Pseudomonas putida | - |
- |
- |
1.14.13.61 | Pseudomonas putida 86 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.61 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.61 | 0.97 | - |
oxygenase component | Pseudomonas putida |
1.14.13.61 | 32.5 | - |
reductase component | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.61 | 2-Oxo-1,2-dihydroquinoline + NADH + O2 | second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline | Pseudomonas putida | ? | - |
? | |
1.14.13.61 | 2-Oxo-1,2-dihydroquinoline + NADH + O2 | second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline | Pseudomonas putida 86 | ? | - |
? | |
1.14.13.61 | 2-Oxo-1,2-dihydroquinoline + NADH + O2 | no catalytic activity under anaerobic conditions | Pseudomonas putida | 8-Hydroxy-2-oxo-1,2-dihydroquinoline + NAD+ + H2O | - |
? | |
1.14.13.61 | 2-Oxo-1,2-dihydroquinoline + NADH + O2 | no catalytic activity under anaerobic conditions | Pseudomonas putida 86 | 8-Hydroxy-2-oxo-1,2-dihydroquinoline + NAD+ + H2O | - |
? | |
1.14.13.61 | additional information | the reductase component shows NADH:acceptor reductase activity with: cytochrome c, ferricyanide, 2,6-dichlorophenol indophenol and 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
1.14.13.61 | additional information | the reductase component shows NADH:acceptor reductase activity with: cytochrome c, ferricyanide, 2,6-dichlorophenol indophenol and 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida 86 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.61 | ? | the reductase component is a monomer, 1 * 37000, SDS-PAGE. The oxygenase component is a hexamer, 6 * 55000, SDS-PAGE | Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.61 | 25 | 30 | - |
Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.61 | 7.5 | - |
- |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.61 | FAD | the reductase component contains 1 FAD | Pseudomonas putida |