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Literature summary extracted from

  • Zheng, Y.; Yu, X.; Li, T.; Xiong, X.; Chen, S.
    Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP+-linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana (2014), Biotechnol. Biofuels, 7, 125-132 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.B64 biofuel production the enzyme might be useful for production biofuels from D-xylose. Chlorella sorokiniana can uptake D-xylose only by an inducible D-xylose transportation system in a light-dependent manner after induction with D-glucose. Xylose reductase (XDH) then converts 50 to 60% of the consumed D-xylose to xylitol, which is subsequently converted to D-xylulose Chlorella sorokiniana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B64 xylitol + NADP+ Chlorella sorokiniana
-
 D-xylulose + NADPH + H+
-
 ?
1.1.1.B64 xylitol + NADP+ Chlorella sorokiniana UTEX 1602
-
 D-xylulose + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B64 Chlorella sorokiniana
-
-
-
1.1.1.B64 Chlorella sorokiniana UTEX 1602
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.B64 0.026
-
 crude enzyme extract, pH 8.0, 40°C, substrates xylitol and NADP+ Chlorella sorokiniana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B64 xylitol + NADP+
-
 Chlorella sorokiniana D-xylulose + NADPH + H+
-
 ?
1.1.1.B64 xylitol + NADP+
-
 Chlorella sorokiniana UTEX 1602 D-xylulose + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B64 NADP+-linked xylitol dehydrogenase
-
 Chlorella sorokiniana
1.1.1.B64 XDH
-
 Chlorella sorokiniana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B64 50
-
-
 Chlorella sorokiniana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.B64 25 65 activity range, profile overview Chlorella sorokiniana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B64 8
-
-
 Chlorella sorokiniana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.B64 6 9 8% of maximal activity at pH 6.0, maximal activity at pH 8.0, and about 50-60% of maximal activity at pH 8.5-9.0, profile overview Chlorella sorokiniana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B64 NADP+ specific for, XDH catalyzes the oxidation of xylitol to D-xylulose only in the presence of the cofactor NADP+ Chlorella sorokiniana

General Information

EC Number General Information Comment Organism
1.1.1.B64 evolution the enzyme occurs exclusively in microorganisms Chlorella sorokiniana