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Literature summary extracted from

  • Nakano, C.; Oshima, M.; Kurashima, N.; Hoshino, T.
    Identification of a new diterpene biosynthetic gene cluster that produces O-methylkolavelool in Herpetosiphon aurantiacus (2015), ChemBioChem, 16, 772-781 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.347 expressed in Escherichia coli BL21(DE3) cells Herpetosiphon aurantiacus
3.1.7.12 expression in Escherichia coili Herpetosiphon aurantiacus
5.5.1.29 expression in Escherichia coli Herpetosiphon aurantiacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.5.1.29 Mg2+ required Herpetosiphon aurantiacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.347 S-adenosyl-L-methionine + (+)-kolavelool Herpetosiphon aurantiacus
-
 S-adenosyl-L-homocysteine + (+)-O-methylkolavelool
-
 ?
2.1.1.347 S-adenosyl-L-methionine + (+)-kolavelool Herpetosiphon aurantiacus ATCC 23779
-
 S-adenosyl-L-homocysteine + (+)-O-methylkolavelool
-
 ?
3.1.7.12 (+)-kolavenyl diphosphate + H2O Herpetosiphon aurantiacus
-
 (+)-kolavelool + diphosphate
-
 ?
3.1.7.12 (+)-kolavenyl diphosphate + H2O Herpetosiphon aurantiacus ATCC 23779
-
 (+)-kolavelool + diphosphate
-
 ?
3.1.7.12 additional information Herpetosiphon aurantiacus enzyme synthesizes (+)-kolavelool through the nucleophilic addition of a water molecule to the incipient cation formed from kolavenyl diphosphate after the diphosphate moiety is released ?
-
 ?
3.1.7.12 additional information Herpetosiphon aurantiacus ATCC 23779 enzyme synthesizes (+)-kolavelool through the nucleophilic addition of a water molecule to the incipient cation formed from kolavenyl diphosphate after the diphosphate moiety is released ?
-
 ?
5.5.1.29 geranylgeranyl diphosphate Herpetosiphon aurantiacus
-
 (+)-kolavenyl diphosphate
-
 ?
5.5.1.29 geranylgeranyl diphosphate Herpetosiphon aurantiacus DSM 785
-
 (+)-kolavenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.347 Herpetosiphon aurantiacus A9AWD7
-
-
2.1.1.347 Herpetosiphon aurantiacus ATCC 23779 A9AWD7
-
-
3.1.7.12 Herpetosiphon aurantiacus A9AWD6
-
-
3.1.7.12 Herpetosiphon aurantiacus ATCC 23779 A9AWD6
-
-
5.5.1.29 Herpetosiphon aurantiacus A9AWD5
-
-
5.5.1.29 Herpetosiphon aurantiacus DSM 785 A9AWD5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.347 Ni-NTA affinity column chromatography Herpetosiphon aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.347 S-adenosyl-L-methionine + (+)-kolavelool
-
 Herpetosiphon aurantiacus S-adenosyl-L-homocysteine + (+)-O-methylkolavelool
-
 ?
2.1.1.347 S-adenosyl-L-methionine + (+)-kolavelool
-
 Herpetosiphon aurantiacus ATCC 23779 S-adenosyl-L-homocysteine + (+)-O-methylkolavelool
-
 ?
3.1.7.12 (+)-kolavenyl diphosphate + H2O
-
 Herpetosiphon aurantiacus (+)-kolavelool + diphosphate
-
 ?
3.1.7.12 (+)-kolavenyl diphosphate + H2O
-
 Herpetosiphon aurantiacus ATCC 23779 (+)-kolavelool + diphosphate
-
 ?
3.1.7.12 additional information enzyme synthesizes (+)-kolavelool through the nucleophilic addition of a water molecule to the incipient cation formed from kolavenyl diphosphate after the diphosphate moiety is released Herpetosiphon aurantiacus ?
-
 ?
3.1.7.12 additional information enzyme synthesizes (+)-kolavelool through the nucleophilic addition of a water molecule to the incipient cation formed from kolavenyl diphosphate after the diphosphate moiety is released Herpetosiphon aurantiacus ATCC 23779 ?
-
 ?
5.5.1.29 geranylgeranyl diphosphate
-
 Herpetosiphon aurantiacus (+)-kolavenyl diphosphate
-
 ?
5.5.1.29 geranylgeranyl diphosphate
-
 Herpetosiphon aurantiacus DSM 785 (+)-kolavenyl diphosphate
-
 ?
5.5.1.29 additional information no substrate: farnesyl diphosphate Herpetosiphon aurantiacus ?
-
 ?
5.5.1.29 additional information no substrate: farnesyl diphosphate Herpetosiphon aurantiacus DSM 785 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.347 Haur_2147 locus name Herpetosiphon aurantiacus
5.5.1.29 Haur_2145
-
 Herpetosiphon aurantiacus

General Information

EC Number General Information Comment Organism
5.5.1.29 physiological function enzyme is a class II diterpene cyclase responsible for the conversion of geranylgeranyl diphosphate into kolavenyl diphosphate. Subsequently, gene product Haur_2146, homologous to Rv3378c, synthesizes (+)-kolavelool through the nucleophilic addition of a water molecule to the incipient cation formed after the diphosphate moiety is released Herpetosiphon aurantiacus