Literature summary extracted from
Garcia, P.; Moreau, A.; Ierich, J.; Vig, A.; Higa, A.; Oliveira, G.; Abdalla, F.; Hausen, M.; Leite, F.
A nanobiosensor based on 4-hydroxyphenylpyruvate dioxygenase enzyme for mesotrione detection (2015), IEEE Sens. J., 15, 2106-2113 .
No PubMed abstract available
Application
EC Number |
Application |
Comment |
Organism |
---|
1.13.11.27 |
diagnostics |
the enzyme can be used for enzyme-based sensors for monitoring herbicides used in agriculture, i.e. mesotrione. Compared to the standard sensors, biosensors have assorted advantages, such as practicality, quick response, low cost, and high sensitivity. A nanobiosensor is developed based on HPPD for mesotrione detection |
Homo sapiens |
1.13.11.27 |
environmental protection |
the enzyme can be used for enzyme-based sensors for monitoring herbicides used in agriculture, i.e. mesotrione. Compared to the standard sensors, biosensors have assorted advantages, such as practicality, quick response, low cost, and high sensitivity. A nanobiosensor is developed based on HPPD for mesotrione detection |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.13.11.27 |
additional information |
construction of a nanobiosensor based on HPPD for mesotrione detection. The atomic force microscope tip functionalization with HPPD immobilized in self-assembled monolayers is confirmed by fluorescence microscopy and atomic force spectroscopy. The cross-linker N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride is responsible for properly preserving the enzyme on the tip |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.13.11.27 |
mesotrione |
molecular docking and molecular dynamics simulation estimate the interacting regions of HPPD with mesotrione using structure PDB ID 3ISQ |
Homo sapiens |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.13.11.27 |
4-hydroxyphenylpyruvate + O2 |
Homo sapiens |
- |
homogentisate + CO2 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.11.27 |
Homo sapiens |
P32754 |
- |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.13.11.27 |
commercial preparation |
pure enzyme |
Homo sapiens |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.11.27 |
4-hydroxyphenylpyruvate + O2 |
- |
Homo sapiens |
homogentisate + CO2 |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.13.11.27 |
HPPD |
- |
Homo sapiens |
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
1.13.11.27 |
0.000068 |
0.0236 |
mesotrione |
dependent on the system used, overview. pH and temperature not specified in the publication |
Homo sapiens |
|