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Literature summary extracted from

  • Ferraro, D.J.; Okerlund, A.; Brown, E.; Ramaswamy, S.
    One enzyme, many reactions structural basis for the various reactions catalyzed by naphthalene 1,2-dioxygenase (2017), IUCrJ, 4, 648-656 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.12 expressed in Escherichia coli DE3 Star cells Pseudomonas sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.12.12 hanging drop vapor diffusion method, using 1.9-2.2 M ammonium sulfate, 4-6% (w/v) dioxane, 100 mM MES, pH 5.0-5.8 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.12 naphthalene + NADH + H+ + O2 Pseudomonas sp.
-
 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
1.14.12.12 naphthalene + NADH + H+ + O2 Pseudomonas sp. C18
-
 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.12 Pseudomonas sp. P0A111
-
-
1.14.12.12 Pseudomonas sp. C18 P0A111
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.12 Q Sepharose column chromatography, octyl Sepharose column chromatography, and hydroxyapatite ion-exchange column chromatography Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.12 1-chloronaphthalene + NADH + H+ + O2
-
 Pseudomonas sp. (1R,2S)-5-chloro-1,2-dihydronaphthalene-1,2-diol + (1S,2R)-8-chloro-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
1.14.12.12 1-chloronaphthalene + NADH + H+ + O2
-
 Pseudomonas sp. C18 (1R,2S)-5-chloro-1,2-dihydronaphthalene-1,2-diol + (1S,2R)-8-chloro-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
1.14.12.12 benzamide + NADH + H+ + O2
-
 Pseudomonas sp. ?
-
 ?
1.14.12.12 benzamide + NADH + H+ + O2
-
 Pseudomonas sp. C18 ?
-
 ?
1.14.12.12 ethylbenzene + NADH + H+ + O2
-
 Pseudomonas sp. (S)-1-phenylethanol + (R)-1-phenylethanol + NAD+
-
 ?
1.14.12.12 ethylbenzene + NADH + H+ + O2
-
 Pseudomonas sp. C18 (S)-1-phenylethanol + (R)-1-phenylethanol + NAD+
-
 ?
1.14.12.12 ethylphenyl sulfide + NADH + H+ + O2
-
 Pseudomonas sp. (R)-ethylphenyl sulfoxide + NAD+
-
 ?
1.14.12.12 ethylphenyl sulfide + NADH + H+ + O2
-
 Pseudomonas sp. C18 (R)-ethylphenyl sulfoxide + NAD+
-
 ?
1.14.12.12 indan + NADH + H+ + O2
-
 Pseudomonas sp. (S)-1-indanol + (R)-1-indanol + NAD+
-
 ?
1.14.12.12 indene + NADH + H+ + O2
-
 Pseudomonas sp. (S)-1-indenol + (R)-1-indenol + NAD+
-
 ?
1.14.12.12 indole-3-acetate + NADH + H+ + O2
-
 Pseudomonas sp. ?
-
 ?
1.14.12.12 naphthalene + NADH + H+ + O2
-
 Pseudomonas sp. (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
1.14.12.12 naphthalene + NADH + H+ + O2
-
 Pseudomonas sp. C18 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
1.14.12.12 phenetole + NADH + H+ + O2
-
 Pseudomonas sp. phenol + vinyloxybenzene + (S)-ethylphenyl sulfoxide + NAD+
-
 ?
1.14.12.12 styrene + NADH + H+ + O2
-
 Pseudomonas sp. (R)-1-phenylethane-1,2-diol + (S)-1-phenylethane-1,2-diol + NAD+
-
 ?
1.14.12.12 thioanisole + NADH + H+ + O2
-
 Pseudomonas sp. methyl phenyl sulfoxide + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.12 NDO
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.12 NADH
-
 Pseudomonas sp.
1.14.12.12 [2Fe-2S]-center
-
 Pseudomonas sp.