Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhang, J.; Li, S.; Xu, H.; Zhou, P.; Zhang, L.; Ouyang, P.
    Purification of xylitol dehydrogenase and improved production of xylitol by increasing XDH activity and NADH supply in Gluconobacter oxydans (2013), J. Agric. Food Chem., 61, 2861-2867 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 gene xyl2, overexpression in Gluconobacter strain PXPG, coexpression with the glucose dehydrogenase gene from Bacillus subtilis Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 xylitol + NAD+ Gluconobacter oxydans
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Gluconobacter oxydans NH-10
-
 D-xylulose + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Gluconobacter oxydans Q8GR61
-
-
1.1.1.9 Gluconobacter oxydans NH-10 Q8GR61
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 additional information enzyme XDH depends exclusively on NAD+/NADH as cofactors Gluconobacter oxydans ?
-
 ?
1.1.1.9 additional information enzyme XDH depends exclusively on NAD+/NADH as cofactors Gluconobacter oxydans NH-10 ?
-
 ?
1.1.1.9 xylitol + NAD+
-
 Gluconobacter oxydans D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Gluconobacter oxydans NH-10 D-xylulose + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 XDH
-
 Gluconobacter oxydans
1.1.1.9 XYL2
-
 Gluconobacter oxydans
1.1.1.9 xylitol dehydrogenase
-
 Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+
-
 Gluconobacter oxydans
1.1.1.9 NADH
-
 Gluconobacter oxydans

Expression

EC Number Organism Comment Expression
1.1.1.9 Gluconobacter oxydans the engineered Gluconobacter oxydans strain PXPG is constructed to coexpress the endogenous XDH gene and the cofactor regeneration glucose dehydrogenase gene from Bacillus subtilis. Activities for both enzymes are more than twofold higher in the Gluconobacter oxydans PXPG than in the wild-type strain. Increasing the XDH activity and the cofactor NADH supply improves the xylitol productivity additional information

General Information

EC Number General Information Comment Organism
1.1.1.9 physiological function enzyme XDH depends exclusively on NAD+/NADH as cofactors with a relatively low activity directly limiting the overall conversion process of D-xylose fermentation to ethanol by Gluconobacter oxydans Gluconobacter oxydans