BRENDA - Enzyme Database show

A tabersonine 3-reductase Catharanthus roseus mutant accumulates vindoline pathway intermediates

Edge, A.; Qu, Y.; Easson, M.L.A.E.; Thamm, A.M.K.; Kim, K.H.; De Luca, V.; Planta 247, 155-169 (2018)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.99.41
expressed in Escherichia coli BL21-(DE3) cells
Catharanthus roseus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.99.41
H189Y
the mutation diminishes the activity of the enzyme by 95%
Catharanthus roseus
1.1.99.41
T305T
inactive
Catharanthus roseus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.99.41
Catharanthus roseus
A0A161CAI1
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.99.41
Ni-NTA column chromatography
Catharanthus roseus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.99.41
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.99.41
16-methoxytabersonine + NADPH + H+
-
743560
Catharanthus roseus
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
-
-
-
?
1.1.99.41
tabersonine + NADPH + H+
-
743560
Catharanthus roseus
2,3-dihydro-3-hydroxytabersonine + NADP+
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.99.41
expressed in Escherichia coli BL21-(DE3) cells
Catharanthus roseus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.99.41
H189Y
the mutation diminishes the activity of the enzyme by 95%
Catharanthus roseus
1.1.99.41
T305T
inactive
Catharanthus roseus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.99.41
Ni-NTA column chromatography
Catharanthus roseus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.99.41
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.99.41
16-methoxytabersonine + NADPH + H+
-
743560
Catharanthus roseus
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
-
-
-
?
1.1.99.41
tabersonine + NADPH + H+
-
743560
Catharanthus roseus
2,3-dihydro-3-hydroxytabersonine + NADP+
-
-
-
?