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Literature summary extracted from

  • Mafu, S.; Karunanithi, P.S.; Palazzo, T.A.; Harrod, B.L.; Rodriguez, S.M.; Mollhoff, I.N.; OBrien, T.E.; Tong, S.; Fiehn, O.; Tantillo, D.J.; Bohlmann, J.; Zerbe, P.
    Biosynthesis of the microtubule-destabilizing diterpene pseudolaric acid B from golden larch involves an unusual diterpene synthase (2017), Proc. Natl. Acad. Sci. USA, 114, 974-979 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.180 expression in Escherichia coli and transient Agrobacterium-mediated expression in Nicotiana benthamiana Pseudolarix amabilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.180 homology modeling and docking of substrate. The Mg2+-coordinating DDxxD motif and arginine residues R558, R560, and R736 critical for catalysis in Abies grandis are conserved in TPS8 Pseudolarix amabilis

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.180 R558A complete loss of activity Pseudolarix amabilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.180 geranylgeranyl diphosphate Pseudolarix amabilis
-
 pseudolaratriene + diphosphate
-
 ?
4.2.3.180 additional information Pseudolarix amabilis no product is formed with geranyl diphosphate or farnesyl diphosphate or in assays with class II diterpene synthases forming copalyl diphosphate of ent- or (+)-stereochemistry ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.180 Pseudolarix amabilis A0A1L6Z3A0
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.180 bark expression predominantly in roots and in bark Pseudolarix amabilis
-
4.2.3.180 needle expression near the limit of detection in needle tissue Pseudolarix amabilis
-
4.2.3.180 root expression predominantly in roots and in bark Pseudolarix amabilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.180 geranylgeranyl diphosphate
-
 Pseudolarix amabilis pseudolaratriene + diphosphate
-
 ?
4.2.3.180 additional information no product is formed with geranyl diphosphate or farnesyl diphosphate or in assays with class II diterpene synthases forming copalyl diphosphate of ent- or (+)-stereochemistry Pseudolarix amabilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.180 pseudolarene synthase
-
 Pseudolarix amabilis
4.2.3.180 TPS8
-
 Pseudolarix amabilis

General Information

EC Number General Information Comment Organism
4.2.3.180 physiological function enzyme catalyzes the first committed step in pseudolaric acid B biosynthesis, showing a distinct carbocation-driven reaction mechanism en route to the 5,7-trans-fused bicyclic pseudolaratriene scaffold Pseudolarix amabilis