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Literature summary extracted from

  • Lin, J.; Milase, R.N.
    Purification and characterization of catechol 1,2-dioxygenase from Acinetobacter sp. Y64 strain and Escherichia coli transformants (2015), Protein J., 34, 421-433 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.1 expressed in Escherichia coli BL21(DE3) cells Acinetobacter sp. Y64

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 Ca2+ 58% residual activity at 0.5 mM Acinetobacter sp. Y64
1.13.11.1 EDTA 50% residual activity at 0.5 mM Acinetobacter sp. Y64
1.13.11.1 additional information not inhibited by Hg2+ Acinetobacter sp. Y64

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.01753
-
 catechol at pH 7.0 and 30°C Acinetobacter sp. Y64

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Fe2+ 144% activity at 0.5 mM Acinetobacter sp. Y64
1.13.11.1 Fe3+ 164% activity at 0.5 mM Acinetobacter sp. Y64

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.1 catechol + O2 Acinetobacter sp. Y64 100% activity cis,cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Acinetobacter sp. Y64
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1 ammonium sulfate precipitation and Hitrap QFF column chromatography Acinetobacter sp. Y64

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 1.42
-
 crude enzyme, at pH 7.0 and 30°C Acinetobacter sp. Y64
1.13.11.1 15.13
-
 afer 10.6fold purification, at pH 7.0 and 30°C Acinetobacter sp. Y64

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 1,2,4-benzenetriol + O2 52% activity compared to catechol Acinetobacter sp. Y64 ?
-
 ?
1.13.11.1 3-methylcatechol + O2 2% activity compared to catechol Acinetobacter sp. Y64 ?
-
 ?
1.13.11.1 4-methylcatechol + O2 61% activity compared to catechol Acinetobacter sp. Y64 ?
-
 ?
1.13.11.1 4-nitrocatechol + O2 80% activity compared to catechol Acinetobacter sp. Y64 ?
-
 ?
1.13.11.1 catechol + O2 100% activity Acinetobacter sp. Y64 cis,cis-muconate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 ? x * 36000, SDS-PAGE Acinetobacter sp. Y64

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 C1,2O
-
 Acinetobacter sp. Y64
1.13.11.1 CatA
-
 Acinetobacter sp. Y64

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 37
-
-
 Acinetobacter sp. Y64

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.1 37 55 the enzyme is stable at the optimal temperature at 37°C for 5 h and loses 10% of its activity after 2 h. As the temperature increases, the enzyme activity declines (37% of activity remains at 45°C under the assay conditions, while the enzyme loses approximately 90% activity in 1 h at 50 and 55°C, respectively) Acinetobacter sp. Y64

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7.7
-
-
 Acinetobacter sp. Y64

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.11.1 0.5
-
 at pH 7.0 and 30°C Acinetobacter sp. Y64 EDTA