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Literature summary extracted from

  • Do, H.; Kim, I.S.; Jeon, B.W.; Lee, C.W.; Park, A.K.; Wi, A.R.; Shin, S.C.; Park, H.; Kim, Y.S.; Yoon, H.S.; Kim, H.W.; Lee, J.H.
    Structural understanding of the recycling of oxidized ascorbate by dehydroascorbate reductase (OsDHAR) from Oryza sativa L. japonica (2016), Sci. Rep., 6, 19498 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.1 expressed in Escherichia coli NiCo21(DE3) cells Oryza sativa Japonica Group

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.5.1 native, ascorbate-bound, and glutathione-bound enzyme forms, hanging drop vapor diffusion method, using 0.15 M potassium bromide and 30% (w/v) PEG MME 2000 Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
1.8.5.1 K20A inactive Oryza sativa Japonica Group
1.8.5.1 K47A the mutant shows about wild type activity Oryza sativa Japonica Group
1.8.5.1 K8A the mutation significantly reduces the enzymatic activity Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.5.1 glutathione + dehydroascorbate Oryza sativa Japonica Group
-
 glutathione disulfide + ascorbate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.1 Oryza sativa Japonica Group Q65XA0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.1 Ni-NTA column chromatography and Superdex 200 gel filtration Oryza sativa Japonica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.1 glutathione + dehydroascorbate
-
 Oryza sativa Japonica Group glutathione disulfide + ascorbate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.5.1 ? x * 23000, SDS-PAGE Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
1.8.5.1 dehydroascorbate reductase
-
 Oryza sativa Japonica Group
1.8.5.1 DHAR
-
 Oryza sativa Japonica Group

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.5.1 30 60 the enzyme retains more than 80% activity between 30 and 60°C for 20 min Oryza sativa Japonica Group