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Literature summary extracted from

  • Han, S.; Pham, T.V.; Kim, J.H.; Lim, Y.R.; Park, H.G.; Jeong, D.; Yun, C.H.; Chun, Y.J.; Kang, L.W.; Kim, D.
    Structural insights into the binding of lauric acid to CYP107L2 from Streptomyces avermitilis (2017), Biochem. Biophys. Res. Commun., 482, 902-908 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.33 gene cyp107l2, recombinant expression of His-tagged enzyme in Escherichia coli Streptomyces avermitilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.15.33 purified recombinant ligand-free CYP107L2 and its complex with lauric acid, (1) sitting drop vapor diffusion method, mixing of 500 nl of 12 mg/ml protein solution with 500 nl of reservoir solution containing 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% w/v PEG 8000, and 15% v/v glycerol, for complex crystals lauric acid in a 1:10 M ratio, and equilibration against 0.05 ml of reservoir solution, 14°C, 30 days, (2) hanging drop vapor diffusion method, mixing of 0.001 ml of 12 mg/ml protein solution with 0.001 ml of reservoir solution containing 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% w/v PEG 8000, and 15% v/v glycerol, and equilibration against 0.05 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.5-2.6 A resolution Streptomyces avermitilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.33 narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 Streptomyces avermitilis
-
 novapikromyin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.15.33 narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 Streptomyces avermitilis ATCC 31267
-
 novapikromyin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.15.33 narbomycin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Streptomyces avermitilis
-
 pikromycin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.33 narbomycin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Streptomyces avermitilis ATCC 31267
-
 pikromycin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.33 Streptomyces avermitilis Q82LM3
-
-
1.14.15.33 Streptomyces avermitilis ATCC 31267 Q82LM3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.33 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Streptomyces avermitilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.33 additional information no catalytic activity with lauric acid. The lauric acid is bound mainly via hydrophobic interactions with the carboxylate group of lauric acid coordinated to the heme of P450. Residue Glu40 and Leu382 in the CYP107L2 complex with lauric acid show significant conformational changes to provide plentiful room for the lauric acid in the substrate-binding site. Binding structure of enzyme, lauric acid and pikromycin, overview Streptomyces avermitilis ?
-
 ?
1.14.15.33 additional information no catalytic activity with lauric acid. The lauric acid is bound mainly via hydrophobic interactions with the carboxylate group of lauric acid coordinated to the heme of P450. Residue Glu40 and Leu382 in the CYP107L2 complex with lauric acid show significant conformational changes to provide plentiful room for the lauric acid in the substrate-binding site. Binding structure of enzyme, lauric acid and pikromycin, overview Streptomyces avermitilis ATCC 31267 ?
-
 ?
1.14.15.33 narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
 Streptomyces avermitilis novapikromyin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.15.33 narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
 Streptomyces avermitilis ATCC 31267 novapikromyin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.15.33 narbomycin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Streptomyces avermitilis pikromycin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.33 narbomycin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Streptomyces avermitilis ATCC 31267 pikromycin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.33 monomer 1 * 43000, recombinant His-tagged enzyme, SDS-PAGE and crystal structure analysis Streptomyces avermitilis

Synonyms

EC Number Synonyms Comment Organism
1.14.15.33 CYP107L2
-
 Streptomyces avermitilis
1.14.15.33 PikC
-
 Streptomyces avermitilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.33 cytochrome P450
-
 Streptomyces avermitilis
1.14.15.33 ferredoxin [iron-sulfur] cluster
-
 Streptomyces avermitilis
1.14.15.33 heme CYP107L2 shows a low-spin state of heme. Heme is sandwiched between helices I and L in the conserved way of P450 structures. The I-helix crosses the center of CYP107L2 in a slightly bent form over the heme structure, while helices F and G are stacked onto the I-helix to form a wide-open substrate-binding cavity just above the heme moiety Streptomyces avermitilis

General Information

EC Number General Information Comment Organism
1.14.15.33 physiological function CYP107L1 catalyzes the hydroxylation of narbomycin to produce pikromycin and novapikromycin Streptomyces avermitilis