EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.1.3 | synthesis | synthesis of 3-hydroxypropionic acid. UTR engineering is used to maximally increase the activities of glycerol dehydratase and aldehyde dehydrogenase for the high conversion of glycerol to 3-hydroxypropionic acid. Thereafter, the activity of glycerol dehydratase is precisely controlled to avoid the accumulation of 3-hydroxypropionaldehyde by varying expression of dhaB1, a gene encoding a main subunit of glycerol dehydratase. The optimally balanced Escherichi coli HGL_DBK4 shows a substantially enhanced 3-hydroxypropionic acid titer and productivity compared with the parental strain. The yield on glycerol is 0.97 g 3-hydroxypropionic acid/g glycerol, in a fed-batch experiment | Azospirillum brasilense |
4.2.1.30 | synthesis | synthesis of 3-hydroxypropionic acid. UTR engineering is used to maximally increase the activities of glycerol dehydratase and aldehyde dehydrogenase for the high conversion of glycerol to 3-hydroxypropionic acid. Thereafter, the activity of glycerol dehydratase is precisely controlled to avoid the accumulation of 3-hydroxypropionaldehyde by varying expression of dhaB1, a gene encoding a main subunit of glycerol dehydratase. The optimally balanced Escherichia coli HGL_DBK4 shows a substantially enhanced 3-hydroxypropionic acid titer and productivity compared with the parental strain. The yield on glycerol is 0.97 g 3-hydroxypropionic acid/g glycerol, in a fed-batch experiment | Klebsiella pneumoniae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.3 | Azospirillum brasilense | - |
- |
- |
4.2.1.30 | Klebsiella pneumoniae | Q59476 AND O08505 AND Q59475 | Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit | - |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.3 | ALDH | - |
Azospirillum brasilense |
4.2.1.30 | GDHt | - |
Klebsiella pneumoniae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.30 | coenzyme B12 | dependent on | Klebsiella pneumoniae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.2.1.3 | metabolism | 3-hydroxypropionic acid production pathway from glycerol | Azospirillum brasilense |
4.2.1.30 | metabolism | 3-hydroxypropionic acid production pathway from glycerol | Klebsiella pneumoniae |