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Literature summary extracted from
- Cloning and characterization of the gene encoding alpha-pinene oxide lyase enzyme (Pralpha-POL) from Pseudomonas rhodesiae CIP 107491 and production of the recombinant protein in Escherichia coli (2018), Appl. Biochem. Biotechnol., 185, 676-690 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.5.1.10 | expressed in Escherichia coli BL21(lambdDE3) cells | Pseudomonas rhodesiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.5.1.10 | alpha-pinene oxide | Pseudomonas rhodesiae | - |
(Z)-2-methyl-5-isopropylhexa-2,5-dienal | - |
? |  |
| 5.5.1.10 | alpha-pinene oxide | Pseudomonas rhodesiae CIP 107491 | - |
(Z)-2-methyl-5-isopropylhexa-2,5-dienal | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.5.1.10 | Pseudomonas rhodesiae | A0A0U2U334 | - |
- |
| 5.5.1.10 | Pseudomonas rhodesiae CIP 107491 | A0A0U2U334 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.5.1.10 | Ni-NTA column chromatography, and Superdex 200 gel filtration | Pseudomonas rhodesiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.5.1.10 | alpha-pinene oxide | - |
Pseudomonas rhodesiae | (Z)-2-methyl-5-isopropylhexa-2,5-dienal | - |
? | |
| 5.5.1.10 | alpha-pinene oxide | - |
Pseudomonas rhodesiae CIP 107491 | (Z)-2-methyl-5-isopropylhexa-2,5-dienal | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.5.1.10 | ? | x * 25000, SDS-PAGE | Pseudomonas rhodesiae |
| 5.5.1.10 | ? | x * 23700, calculated from amino acid sequence | Pseudomonas rhodesiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.5.1.10 | alpha-pinene oxide lyase | - |
Pseudomonas rhodesiae |
| 5.5.1.10 | Pralpha-POL | - |
Pseudomonas rhodesiae |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 5.5.1.10 | Pseudomonas rhodesiae | calculated from amino acid sequence | - |
5.2 |
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