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Literature summary extracted from

  • Pemberton, T.A.; Chen, M.; Harris, G.G.; Chou, W.K.; Duan, L.; Koeksal, M.; Genshaft, A.S.; Cane, D.E.; Christianson, D.W.
    Exploring the influence of domain architecture on the catalytic function of diterpene synthases (2017), Biochemistry, 56, 2010-2023 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.17 expressed in Escherichia coli BL21(DE3)RIL CodonPlus cells Taxus brevifolia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.17 0.0029
-
 geranylgeranyl diphosphate full-length enzyme, at pH 8.0 and 30°C Taxus brevifolia
4.2.3.43 0.00063
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.17 geranylgeranyl diphosphate Taxus brevifolia
-
 taxa-4,11-diene + diphosphate
-
 ?
4.2.3.43 geranylgeranyl diphosphate Diaporthe amygdali
-
 fusicocca-2,10(14)-diene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.17 Taxus brevifolia
-
-
-
4.2.3.43 Diaporthe amygdali
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.17 Ni-NTA column chromatography Taxus brevifolia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.17 geranylgeranyl diphosphate
-
 Taxus brevifolia taxa-4,11-diene + diphosphate
-
 ?
4.2.3.43 geranylgeranyl diphosphate
-
 Diaporthe amygdali fusicocca-2,10(14)-diene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.43 fusicoccadiene synthase
-
 Diaporthe amygdali

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.17 0.16
-
 geranylgeranyl diphosphate full-length enzyme, at pH 8.0 and 30°C Taxus brevifolia
4.2.3.43 0.021
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.17 55
-
 geranylgeranyl diphosphate full-length enzyme, at pH 8.0 and 30°C Taxus brevifolia
4.2.3.43 33
-
 geranylgeranyl diphosphate pH and temperature not specified in the publication Diaporthe amygdali