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Literature summary extracted from

  • Steiner, E.M.; Boeth, D.; Loessl, P.; Vilaplana, F.; Schnell, R.; Schneider, G.
    CysK2 from Mycobacterium tuberculosis is an O-phospho-L-serine-dependent S-sulfocysteine synthase (2014), J. Bacteriol., 196, 3410-3420 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.5.1 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.8.5.1 R243A the mutant shows about 10% activity with sulfide compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.5.1 0.0439
-
 thiosulfate wild type enzyme, at pH 9.0 and 22°C Mycobacterium tuberculosis
2.8.5.1 0.2137
-
 thiosulfate mutant enzyme R243A, at pH 9.0 and 22°C Mycobacterium tuberculosis
2.8.5.1 0.4851
-
 3-phospho-L-serine mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C Mycobacterium tuberculosis
2.8.5.1 1.0855
-
 3-phospho-L-serine wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.5.1 3-phospho-L-serine + thiosulfate Mycobacterium tuberculosis
-
 S-sulfo-L-cysteine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.5.1 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.5.1 Ni-NTA column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.5.1 3-phospho-L-serine + sulfide
-
 Mycobacterium tuberculosis ?
-
 ?
2.8.5.1 3-phospho-L-serine + thiosulfate
-
 Mycobacterium tuberculosis S-sulfo-L-cysteine + phosphate
-
 ?
2.8.5.1 3-phospho-L-serine + thiosulfate highest activity Mycobacterium tuberculosis S-sulfo-L-cysteine + phosphate
-
 ?
2.8.5.1 additional information the enzyme does not utilize thiocarboxylated CysO as a sulfur donor. O-acetyl-L-serine, L-Asp, L-Val, L-Gln, L-Glu, L-Ser, L-Asn, L-Cys, L-Ser, L-Leu, homocysteine, pyruvate, 2-oxoglutarate, 3-phosphoglycerate, succinate, N-acetylcysteine and diaminopimelic acid are not used as substrate Mycobacterium tuberculosis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.5.1 CysK2
-
 Mycobacterium tuberculosis
2.8.5.1 O-phosphoserine S-sulfocysteine synthase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.5.1 0.457
-
 thiosulfate mutant enzyme R243A, at pH 9.0 and 22°C Mycobacterium tuberculosis
2.8.5.1 0.457
-
 3-phospho-L-serine mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C Mycobacterium tuberculosis
2.8.5.1 0.913
-
 thiosulfate wild type enzyme, at pH 9.0 and 22°C Mycobacterium tuberculosis
2.8.5.1 0.913
-
 3-phospho-L-serine wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.5.1 9
-
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.5.1 pyridoxal 5'-phosphate
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8.5.1 0.000083
-
 3-phospho-L-serine wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C Mycobacterium tuberculosis
2.8.5.1 0.000093
-
 3-phospho-L-serine mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C Mycobacterium tuberculosis
2.8.5.1 0.0021
-
 thiosulfate mutant enzyme R243A, at pH 9.0 and 22°C Mycobacterium tuberculosis
2.8.5.1 0.0208
-
 thiosulfate wild type enzyme, at pH 9.0 and 22°C Mycobacterium tuberculosis