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Literature summary extracted from

  • Del Prete, S.; Vullo, D.; De Luca, V.; Supuran, C.T.; Capasso, C.
    Biochemical characterization of the delta-carbonic anhydrase from the marine diatom Thalassiosira weissflogii, TweCA (2014), J. Enzyme Inhib. Med. Chem., 29, 906-911 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 gene ca1, encoding delta-CA, sequence comparisons, phylogenetic analysis, recombinant His-tagged enzyme expression in Escherichia coli Conticribra weissflogii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ required, metalloenzyme Conticribra weissflogii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Conticribra weissflogii
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Conticribra weissflogii Q5U9J1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatagraphy Conticribra weissflogii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Conticribra weissflogii H2CO3
-
 r
4.2.1.1 H2CO3
-
 Conticribra weissflogii CO2 + H2O
-
 r
4.2.1.1 additional information enzyme TweCA has no esterase activity with 4-nitrophenyl acetate as substrate, measurement of CO2 hydration reaction for determination of enzyme activity by stopped-flow pH-based assay with bromothymol blue as indicator Conticribra weissflogii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 delta carbonic anhydrase
-
 Conticribra weissflogii
4.2.1.1 delta-carbonic anhydrase
-
 Conticribra weissflogii
4.2.1.1 delta-class CA
-
 Conticribra weissflogii
4.2.1.1 TweCA
-
 Conticribra weissflogii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1
-
-
 assay at Conticribra weissflogii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.1 80
-
 purified recombinant His-tagged enzyme, pH 8.3, 40% activity remaining after 30 min, complete inactivation after 60 min Conticribra weissflogii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 130000
-
 CO2 measured with hydration of CO2 activity assay, pH 7.5-8.3, 0°C Conticribra weissflogii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 7.5 8.3 assay at Conticribra weissflogii

General Information

EC Number General Information Comment Organism
4.2.1.1 evolution several genetically distinct classes of carbonic anhydrase (CAs) exist, e.g. alpha-, beta-, gamma-, delta- and zeta-, and all of them are metalloenzymes. delta-CAs are closer to the alpha-CAs than to the other classes of the enzymes, phylogenetic analysis Conticribra weissflogii
4.2.1.1 physiological function carbonic anhydrases are key enzymes involved in the acquisition of inorganic carbon for photosynthesis in phytoplankton, as they catalyze efficiently the interconversion between carbon dioxide and bicarbonate Conticribra weissflogii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.1 33000
-
 CO2 measured with hydration of CO2 activity assay, pH 7.5-8.3, 0°C Conticribra weissflogii