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Literature summary extracted from

  • Eprintsev, A.T.; Fedorin, D.N.; Salnikov, A.V.; Igamberdiev, A.U.
    Expression and properties of the glyoxysomal and cytosolic forms of isocitrate lyase in Amaranthus caudatus L (2015), J. Plant Physiol., 181, 1-8 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 Cu2+ isoforms ICL1 and ICL2 are 60-80% inhibited by 5 mM Cu2+ Amaranthus caudatus
4.1.3.1 Fe2+ isoforms ICL1 and ICL2 are 30% inhibited by 5 mM Fe2+ Amaranthus caudatus
4.1.3.1 Mg2+ isoform ICL2 is 20% inhibited by 5 mM Mg2+ Amaranthus caudatus
4.1.3.1 Mn2+ isoform ICL1 is 40% inhibited by 5 mM Mn2+ Amaranthus caudatus
4.1.3.1 Zn2+ isoforms ICL1 and ICL2 are 60-80% inhibited by 5 mM Zn2+ Amaranthus caudatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.045
-
 DL-isocitrate isoform ICL1, with 5 mM MgCl2, at pH 7.5 and 25°C Amaranthus caudatus
4.1.3.1 0.063
-
 DL-isocitrate isoform ICL2, with 5 mM MgCl2, at pH 7.5 and 25°C Amaranthus caudatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.3.1 cytosol isoform ICL2 Amaranthus caudatus 5829
-
4.1.3.1 glyoxysome isoform ICL1 Amaranthus caudatus 9514
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Mg2+ isoform ICL1 is activated 3fold by 5 mM Mg2+ Amaranthus caudatus
4.1.3.1 Mn2+ isoform ICL2 is activated 3fold by 5 mM Mn2+ Amaranthus caudatus
4.1.3.1 additional information isoform ICL2 is not activated by Mg2+. Ca2+and K+ reveal no marked effect on both isoforms of the enzyme Amaranthus caudatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Amaranthus caudatus
-
 succinate + glyoxylate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Amaranthus caudatus
-
 cultivar Kinelskiy 254
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-25 gel filtration Amaranthus caudatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.1 seedling
-
 Amaranthus caudatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 0.024
-
 isoform ICL2, at pH 6.5 and 25°C Amaranthus caudatus
4.1.3.1 0.03
-
 isoform ICL1, with 5 mM MnCl2, at pH 6.5 and 25°C Amaranthus caudatus
4.1.3.1 0.035
-
 isoform ICL2, with 5 mM MnCl2, at pH 6.5 and 25°C Amaranthus caudatus
4.1.3.1 0.48
-
 isoform ICL1, at pH 6.5 and 25°C Amaranthus caudatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 DL-isocitrate
-
 Amaranthus caudatus succinate + glyoxylate
-
 r
4.1.3.1 isocitrate
-
 Amaranthus caudatus succinate + glyoxylate
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 D-threo-isocitrate: glyoxylate lyase
-
 Amaranthus caudatus
4.1.3.1 ICL1 isoform Amaranthus caudatus
4.1.3.1 ICL2 isoform Amaranthus caudatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 6.5
-
 isoform ICL2 Amaranthus caudatus
4.1.3.1 7.5
-
 isoform ICL1 Amaranthus caudatus

Expression

EC Number Organism Comment Expression
4.1.3.1 Amaranthus caudatus the highest isoform ICL1 activity is observed on the 3rd day of germination; then the activity and expression of the corresponding gene decreases, while the activity of isoform ICL2 and gene expression increases to the 7th day of germination and then remains at the same level up

General Information

EC Number General Information Comment Organism
4.1.3.1 metabolism the function of isoform ICL1 is related to the glyoxylate cycle while isoform ICL2 functions independently from the glyoxylate cycle and interconverts organic acids in the cytosol Amaranthus caudatus