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Literature summary extracted from

  • Park, Y.; Cho, Y.; Lee, Y.H.; Lee, Y.W.; Rhee, S.
    Crystal structure and functional analysis of isocitrate lyases from Magnaporthe oryzae and Fusarium graminearum (2016), J. Struct. Biol., 194, 395-403 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.1 expressed in Escherichia coli BL21(DE3) cells Fusarium graminearum
4.1.3.1 expressed in Escherichia coli BL21(DE3) cells Pyricularia oryzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.1 complexed with Mn2+ and malonate, sitting drop vapor diffusion method, using 2.4 M sodium malonate Fusarium graminearum
4.1.3.1 ligand-free form and as a complex with Mg2+, glyoxylate, and glycerol, sitting drop vapor diffusion method, using 0.2 M sodium malonate and 20% (w/v) PEG 3350 or 0.2 M potassium sodium tartrate and 10% (v/v) glycerol Pyricularia oryzae

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.1 C215A inactive Pyricularia oryzae
4.1.3.1 C215S inactive Pyricularia oryzae
4.1.3.1 E206A inactive Pyricularia oryzae
4.1.3.1 E405A inactive Pyricularia oryzae
4.1.3.1 N433A inactive Pyricularia oryzae
4.1.3.1 R252A inactive Pyricularia oryzae
4.1.3.1 R252K inactive Pyricularia oryzae
4.1.3.1 T524V the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Pyricularia oryzae
4.1.3.1 T534A the mutant shows reduced Km and kcat values compared to the wild type enzyme Pyricularia oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.5287
-
 isocitrate wild type enzyme, at pH 7.0 and 37°C Pyricularia oryzae
4.1.3.1 0.5287
-
 isocitrate mutant enzyme T534A, at pH 7.0 and 37°C Pyricularia oryzae
4.1.3.1 0.5433
-
 isocitrate mutant enzyme T534V, at pH 7.0 and 37°C Pyricularia oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Fusarium graminearum
-
 succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate Pyricularia oryzae
-
 succinate + glyoxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Fusarium graminearum Q4HYR2
-
-
4.1.3.1 Pyricularia oryzae P0CT06
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 Ni-NTA column chromatography and Superdex 200 gel filtration Fusarium graminearum
4.1.3.1 Ni-NTA column chromatography and Superdex 200 gel filtration Pyricularia oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Fusarium graminearum succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Pyricularia oryzae succinate + glyoxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 homotetramer x-ray crystallography Fusarium graminearum
4.1.3.1 homotetramer x-ray crystallography Pyricularia oryzae

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 ICL
-
 Fusarium graminearum
4.1.3.1 ICL
-
 Pyricularia oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.1 4.4
-
 isocitrate mutant enzyme T534A, at pH 7.0 and 37°C Pyricularia oryzae
4.1.3.1 15.9
-
 isocitrate mutant enzyme T534V, at pH 7.0 and 37°C Pyricularia oryzae
4.1.3.1 17.6
-
 isocitrate wild type enzyme, at pH 7.0 and 37°C Pyricularia oryzae