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Literature summary extracted from

  • Ignea, C.; Trikka, F.; Nikolaidis, A.; Georgantea, P.; Ioannou, E.; Loupassaki, S.; Kefalas, P.; Kanellis, A.; Roussis, V.; Makris, A.; Kampranis, S.
    Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase (2015), Metab. Eng., 27, 65-75 .
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.3.32 synthesis the development of yeast strains carrying the engineered Erg20p, which support efficient isoprenoid production, e.g. by abietadiene synthase, and can be used as a dedicated chassis for diterpene production or biosynthetic pathway elucidation. The design developed can be applied to the production of any GGPP-derived isoprenoid and is compatible with other yeast terpene production platforms, method overview Picea abies
5.5.1.12 synthesis the development of yeast strains carrying the engineered Erg20p, which support efficient isoprenoid production, e.g. by abietadiene synthase, and can be used as a dedicated chassis for diterpene production or biosynthetic pathway elucidation. The design developed can be applied to the production of any GGPP-derived isoprenoid and is compatible with other yeast terpene production platforms, method overview Picea abies

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.32 gene TPS-LAS, recombinant expression in Saccharomyces cerevisiae strain AM94, a yeast strain engineered for the production of isoprenoids, which contains three chromosomally integrated copies of a degradation stabilized variant of HMG2 and a heterozygous deletion of ERG9, and in strain AM204, each with or without coexpression of yeast protein Erg20p(F96C), a yeast farnesyl diphosphate synthase, overview. Coexpression with different Saccharomyces cerevisiae ERG20 variants increases diterpene yields by up to 15fold compared to expression of the Cistus creticus GGPPS Picea abies
5.5.1.12 gene TPS-LAS, recombinant expression in Saccharomyces cerevisiae strain AM94, a yeast strain engineered for the production of isoprenoids, which contains three chromosomally integrated copies of a degradation stabilized variant of HMG2 and a heterozygous deletion of ERG9, and in strain AM204, each with or without coexpression of yeast protein Erg20p(F96C), a yeast FPP synthase, overview Picea abies

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.32 additional information using a combination of protein and genetic engineering, significant improvements in the production of sclareol and several other isoprenoids is achieved, including cis-abienol, abietadiene, and beta-carotene. Recombinant expression of enzyme PaLAS in Saccharomyces cerevisiae AM94 cells, coexpression with different Saccharomyces cerevisiae farnesyl diphosphate synthase ERG20 variants increases diterpene yields by up to 15fold compared to expression of the Cistus creticus GGPPS Picea abies
5.5.1.12 additional information using a combination of protein and genetic engineering, significant improvements in the production of sclareol and several other isoprenoids is achieved, including cis-abienol, abietadiene, and beta-carotene. Recombinant expression of enzyme PaLAS in Saccharomyces cerevisiae AM94 cells, coexpression with different Saccharomyces cerevisiae farnesyl diphosphate synthase ERG20 variants increases diterpene yields by up to 15fold compared to expression of the Cistus creticus GGPPS Picea abies

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.32 chloroplast
-
 Picea abies 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.32 Mg2+ required Picea abies
5.5.1.12 Mg2+ required Picea abies

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.32 (+)-copalyl diphosphate Picea abies
-
 abieta-8(14),12-diene + diphosphate
-
 ?
4.2.3.32 additional information Picea abies abietadiene synthase (PaLAS) is a bifunctional terpene synthase that catalyzes both class I and class II reactions, i.e. formation of (+)-copalyl diphosphate from geranylgeranyl diphosphate (cf. EC 5.5.1.12, class II diTPS), and formation of abieta-8(14),12-diene from (+)-copalyl diphosphate (EC 4.2.3.32, class I diTPS) ?
-
 ?
5.5.1.12 geranylgeranyl diphosphate Picea abies
-
 (+)-copalyl diphosphate
-
 ?
5.5.1.12 additional information Picea abies abietadiene synthase (PaLAS) is a bifunctional terpene synthase that catalyzes both class I and class II reactions, i.e. formation of (+)-copalyl diphosphate from geranylgeranyl diphosphate (EC 5.5.1.12, class II diTPS), and formation of abieta-8(14),12-diene from (+)-copalyl diphosphate (cf. EC 4.2.3.32, class I diTPS) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.32 Picea abies Q675L4
-
-
5.5.1.12 Picea abies Q675L4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.32 (+)-copalyl diphosphate
-
 Picea abies abieta-8(14),12-diene + diphosphate
-
 ?
4.2.3.32 additional information abietadiene synthase (PaLAS) is a bifunctional terpene synthase that catalyzes both class I and class II reactions, i.e. formation of (+)-copalyl diphosphate from geranylgeranyl diphosphate (cf. EC 5.5.1.12, class II diTPS), and formation of abieta-8(14),12-diene from (+)-copalyl diphosphate (EC 4.2.3.32, class I diTPS) Picea abies ?
-
 ?
5.5.1.12 geranylgeranyl diphosphate
-
 Picea abies (+)-copalyl diphosphate
-
 ?
5.5.1.12 additional information abietadiene synthase (PaLAS) is a bifunctional terpene synthase that catalyzes both class I and class II reactions, i.e. formation of (+)-copalyl diphosphate from geranylgeranyl diphosphate (EC 5.5.1.12, class II diTPS), and formation of abieta-8(14),12-diene from (+)-copalyl diphosphate (cf. EC 4.2.3.32, class I diTPS) Picea abies ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.32 abietadiene synthase
-
 Picea abies
4.2.3.32 More cf. EC 5.5.1.12 Picea abies
4.2.3.32 PaLAS
-
 Picea abies
4.2.3.32 TPS-LAS
-
 Picea abies
5.5.1.12 abietadiene synthase
-
 Picea abies
5.5.1.12 More cf. EC 4.2.3.32 Picea abies
5.5.1.12 PaLAS
-
 Picea abies
5.5.1.12 TPS-LAS
-
 Picea abies

General Information

EC Number General Information Comment Organism
4.2.3.32 evolution the bifunctional enzyme belongs to the class I and II diterpene synthases Picea abies
5.5.1.12 evolution the bifunctional enzyme belongs to the class I and II diterpene synthases Picea abies