BRENDA - Enzyme Database

Expression and purification of imidazole glycerol phosphate synthase from Saccharomyces cerevisiae

Chittur, S.V.; Chen, Y.; Davisson, V.J.; Protein Expr. Purif. 18, 366-377 (2000)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
4.3.2.10
catalytically active yeast HIS7 is expressed in Escherichia coli under the control of T7 polymerase promoter
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.2.10
0.004
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
1.7
-
L-glutamine
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
246
-
NH4+
pH 7.0, 30C
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.2.10
55000
-
gel filtration
Saccharomyces cerevisiae
4.3.2.10
57000
-
equilibrium sedimentation
Saccharomyces cerevisiae
4.3.2.10
61000
-
SDS-PAGE
Saccharomyces cerevisiae
4.3.2.10
61003
-
MALDI MS
Saccharomyces cerevisiae
4.3.2.10
61082
-
calculated from sequence
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Saccharomyces cerevisiae
the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Saccharomyces cerevisiae ATCC 204508
the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
4.3.2.10
Saccharomyces cerevisiae
P33734
-
-
4.3.2.10
Saccharomyces cerevisiae ATCC 204508
P33734
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
4.3.2.10
recombinant enzyme
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
4.3.2.10
-80C, stored without loss of activity
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
749228
Saccharomyces cerevisiae
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis
749228
Saccharomyces cerevisiae
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
749228
Saccharomyces cerevisiae ATCC 204508
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis
749228
Saccharomyces cerevisiae ATCC 204508
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+
the yeast enzyme is distinguished from the Escherichia coli IGP synthase in its utilization of ammonia as a substrate
749228
Saccharomyces cerevisiae
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+
the yeast enzyme is distinguished from the Escherichia coli IGP synthase in its utilization of ammonia as a substrate
749228
Saccharomyces cerevisiae ATCC 204508
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.3.2.10
monomer
1 * 61000, SDS-PAGE
Saccharomyces cerevisiae
4.3.2.10
monomer
1 * 61082, calculated from sequence
Saccharomyces cerevisiae
Synonyms
EC Number
Synonyms
Commentary
Organism
4.3.2.10
HIS7
-
Saccharomyces cerevisiae
4.3.2.10
IGP synthase
-
Saccharomyces cerevisiae
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.2.10
0.6
-
NH4+
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
3.9
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
5.2
-
L-glutamine
pH 7.0, 30C
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.2.10
7
-
glutamine-dependent activity
Saccharomyces cerevisiae
4.3.2.10
7
8.5
NH4+-dependent activity
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.2.10
catalytically active yeast HIS7 is expressed in Escherichia coli under the control of T7 polymerase promoter
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.2.10
0.004
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
1.7
-
L-glutamine
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
246
-
NH4+
pH 7.0, 30C
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.2.10
55000
-
gel filtration
Saccharomyces cerevisiae
4.3.2.10
57000
-
equilibrium sedimentation
Saccharomyces cerevisiae
4.3.2.10
61000
-
SDS-PAGE
Saccharomyces cerevisiae
4.3.2.10
61003
-
MALDI MS
Saccharomyces cerevisiae
4.3.2.10
61082
-
calculated from sequence
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Saccharomyces cerevisiae
the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Saccharomyces cerevisiae ATCC 204508
the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.2.10
recombinant enzyme
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.3.2.10
-80C, stored without loss of activity
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
749228
Saccharomyces cerevisiae
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis
749228
Saccharomyces cerevisiae
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
749228
Saccharomyces cerevisiae ATCC 204508
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis
749228
Saccharomyces cerevisiae ATCC 204508
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+
the yeast enzyme is distinguished from the Escherichia coli IGP synthase in its utilization of ammonia as a substrate
749228
Saccharomyces cerevisiae
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
-
-
?
4.3.2.10
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + NH4+
the yeast enzyme is distinguished from the Escherichia coli IGP synthase in its utilization of ammonia as a substrate
749228
Saccharomyces cerevisiae ATCC 204508
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.2.10
monomer
1 * 61000, SDS-PAGE
Saccharomyces cerevisiae
4.3.2.10
monomer
1 * 61082, calculated from sequence
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.2.10
0.6
-
NH4+
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
3.9
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
5.2
-
L-glutamine
pH 7.0, 30C
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.2.10
7
-
glutamine-dependent activity
Saccharomyces cerevisiae
4.3.2.10
7
8.5
NH4+-dependent activity
Saccharomyces cerevisiae
General Information
EC Number
General Information
Commentary
Organism
4.3.2.10
metabolism
the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis
Saccharomyces cerevisiae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.3.2.10
metabolism
the enzyme represents a junction between histidine biosynthesis and de novo purine biosynthesis
Saccharomyces cerevisiae
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.3.2.10
0.024
-
NH4+
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
3.1
-
L-glutamine
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
950
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
pH 7.0, 30C
Saccharomyces cerevisiae
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.3.2.10
0.024
-
NH4+
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
3.1
-
L-glutamine
pH 7.0, 30C
Saccharomyces cerevisiae
4.3.2.10
950
-
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
pH 7.0, 30C
Saccharomyces cerevisiae