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Literature summary extracted from

  • Ge, L.; Chen, A.; Pei, J.; Zhao, L.; Fang, X.; Ding, G.; Wang, Z.; Xiao, W.; Tang, F.
    Enhancing the thermostability of alpha-L-rhamnosidase from Aspergillus terreus and the enzymatic conversion of rutin to isoquercitrin by adding sorbitol (2017), BMC Biotechnol., 17, 21 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.40 industry stable alpha-L-rhamnosidase with cleaving terminal alpha-L-rhamnose activity has great potential in industrial application. Enhancing the thermostability of alpha-L-rhamnosidase from Aspergillus terreus makes it a good candidate for industrial processes of isoquercitrin preparation Aspergillus terreus
3.2.1.40 synthesis adding sorbitol has great potential to promote enzymatic conversion of rutin to isoquercitrin production. Isoquercitrin has several biological activities, including anti-mutagenesis, anti-virus, anti-hypertensive, anti-proliferative effects, lipid peroxidation, oxidative-stress protection as well as other pharmacological effects Aspergillus terreus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.40 expression in Pichia pastoris Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 0.481
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.5, 65°C, without additives Aspergillus terreus
3.2.1.40 0.493
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.5, 65°C, in presence of 0.6 M sorbitol Aspergillus terreus
3.2.1.40 0.542
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.5, 65°C, in presence of 1.0 M sorbitol Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Aspergillus terreus I0AZ41
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Aspergillus terreus 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 rutin + H2O
-
 Aspergillus terreus isoquercitrin + alpha-L-rhamnose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 65
-
 half-life: 127.9 min without sorbitol, 5803 min in presence of 2.0 M sorbitol Aspergillus terreus
3.2.1.40 70
-
 half-life: 18.2 min without sorbitol, 313.7 min in presence of 2.0 M sorbitol. Half-life and activation free energy are increased by 17.2fold and 8.2 kJ/mol, respectively in presence of 2.0 M sorbitol Aspergillus terreus
3.2.1.40 75
-
 half-life: 1.8 min without sorbitol, 54.6 min in presence of 2.0 M sorbitol. 2.0 M sorbitol prolonges the half-life 30.3fold Aspergillus terreus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.40 362.5
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.5, 65°C, in presence of 1.0 M sorbitol Aspergillus terreus
3.2.1.40 379.2
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.5, 65°C, in presence of 0.6 M sorbitol Aspergillus terreus
3.2.1.40 407.6
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.5, 65°C, without additives Aspergillus terreus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.40 670
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.5, 65°C, in presence of 1.0 M sorbitol Aspergillus terreus
3.2.1.40 770
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.5, 65°C, in presence of 0.6 M sorbitol Aspergillus terreus
3.2.1.40 850
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 6.5, 65°C, without additives Aspergillus terreus