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Literature summary extracted from

  • Shehata, A.; Darwish, D.; Masoud, H.
    Extraction, purification and characterization of endo-acting pullulanase type I from white edible mushrooms (2016), J. Appl. Pharm. Sci., 6, 147-152 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.41 dithiothreitol 1 M, 1.1fold activation unidentified
3.2.1.41 EDTA 1 M, 1.3fold activation unidentified

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 Ag+ 0.1 M, 50% loss of activity unidentified
3.2.1.41 Hg2+ 0.1 M, 70% loss of activity unidentified
3.2.1.41 iodoacetate 1 M, 10% loss of activity unidentified
3.2.1.41 Sodium fluoride 1 M, 10% loss of activity unidentified

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.41 0.27
-
 pullulan pH 6, 40°C unidentified

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 Ca2+ 0.1 M, 1.4fold activation unidentified
3.2.1.41 Fe2+ 0.1 M, 1.6fold activation unidentified
3.2.1.41 Mn2+ 0.1 M, 1.5fold activation unidentified

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 112000
-
 SDS-PAGE unidentified

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 unidentified
-
 edible white mushrooms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41
-
 unidentified

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O hydrolyzed at 60% compared to pullulan unidentified ?
-
 ?
3.2.1.41 glycogen + H2O hydrolyzed at 30% compared to pullulan unidentified ?
-
 ?
3.2.1.41 pullulan + H2O
-
 unidentified maltotriose + ?
-
 ?
3.2.1.41 soluble starch + H2O hydrolyzed at 80% compared to pullulan unidentified ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 ? x * 112000, SDS-PAGE unidentified

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 40
-
-
 unidentified

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.41 30 70 30°C: about 60% of maximal activity, 70°C: about 40% of maximal activity unidentified

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 60
-
 30 min, 50% loss of activity unidentified
3.2.1.41 70
-
 45 min, 80% loss of activity unidentified

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6
-
-
 unidentified

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.41 5 8 pH 5.0: about 30% of maximal activity, pH 8.0: about 30% of maximal activity unidentified

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.41 unidentified isoelectric focusing
-
 6.2