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Literature summary extracted from

  • Hromic-Jahjefendic, A.; Jajcanin Jozic, N.; Kazazic, S.; Grabar Branilovic, M.; Karacic, Z.; Schrittwieser, J.H.; Das, K.M.P.; Tomin, M.; Oberer, M.; Gruber, K.; Abramic, M.; Tomic, S.
    A novel Porphyromonas gingivalis enzyme An atypical dipeptidyl peptidase III with an ARM repeat domain (2017), PLoS ONE, 12, e0188915 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.4 0.00097
-
 Arg-Arg-2-naphthylamide pH 8.0, 37°C Porphyromonas gingivalis
3.4.14.4 0.00256
-
 Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Porphyromonas gingivalis

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.4 Porphyromonas gingivalis Q7MX92
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.4 Ala-Arg-2-naphthylamide + H2O
-
 Porphyromonas gingivalis Ala-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Arg-Arg-2-naphthylamide + H2O
-
 Porphyromonas gingivalis Arg-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Porphyromonas gingivalis Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.14.4 DRVYIHPF + H2O i.e. angiotensin II Porphyromonas gingivalis IHPF + ?
-
 ?
3.4.14.4 IVYPW + H2O
-
 Porphyromonas gingivalis YPW + ?
-
 ?
3.4.14.4 Phe-Arg-2-naphthylamide + H2O
-
 Porphyromonas gingivalis Phe-Arg + 2-naphthylamine
-
 ?
3.4.14.4 VVYPW + H2O i.e. tynorphin Porphyromonas gingivalis YPW + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.4 ? x * 102000, SDS-PAGE Porphyromonas gingivalis

Synonyms

EC Number Synonyms Comment Organism
3.4.14.4 PG_0317
-
 Porphyromonas gingivalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.4 0.29
-
 Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Porphyromonas gingivalis
3.4.14.4 0.75
-
 Arg-Arg-2-naphthylamide pH 8.0, 37°C Porphyromonas gingivalis

General Information

EC Number General Information Comment Organism
3.4.14.4 physiological function the enzyme possesses a C-terminal extension containing an Armadillo type fold similar to the AlkD family of bacterial DNA glycosylases. The Km values for both Arg-Arg substrates are increased by 4fold when the C-terminal domain is removed. kcat values are reduced 120fold in the case of Arg-Arg-4-methylcoumarin 7-amide, and 250fold in the case of Arg-Arg-2-naphthylamide, resulting in a 470fold and 1050fold reduction in the catalytic efficiency, respectively. Complementation assays in a DNA repair-deficient Escherichia coli strain indicate the absence of alkylation repair function Porphyromonas gingivalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.14.4 113
-
 Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Porphyromonas gingivalis
3.4.14.4 773
-
 Arg-Arg-2-naphthylamide pH 8.0, 37°C Porphyromonas gingivalis