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Literature summary extracted from

  • Tian, Y.; Xu, W.; Zhang, W.; Zhang, T.; Guang, C.; Mu, W.
    Amylosucrase as a transglucosylation tool From molecular features to bioengineering applications (2018), Biotechnol. Adv., 36, 1540-1552 .
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.4 Alteromonas macleodii B6F2H1
-
-
2.4.1.4 Cellulomonas carbonis A0A0A0BUC7
-
-
2.4.1.4 Cellulomonas carbonis T26 A0A0A0BUC7
-
-
2.4.1.4 Deinococcus geothermalis Q1J0W0
-
-
2.4.1.4 Deinococcus geothermalis DSM 11300 Q1J0W0
-
-
2.4.1.4 Deinococcus radiodurans Q9RVT9
-
-
2.4.1.4 Deinococcus radiodurans ATCC 13939 Q9RVT9
-
-
2.4.1.4 Deinococcus radiopugnans
-
-
-
2.4.1.4 Methylobacillus flagellatus Q1GY12
-
-
2.4.1.4 Methylotuvimicrobium alcaliphilum G4T024
-
-
2.4.1.4 Methylotuvimicrobium alcaliphilum DSM 19304 G4T024
-
-
2.4.1.4 Neisseria polysaccharea Q9ZEU2
-
-
2.4.1.4 Neisseria subflava D3A730
-
-
2.4.1.4 Neisseria subflava NJ9703 D3A730
-
-
2.4.1.4 Pseudarthrobacter chlorophenolicus B8H6N5
-
-
2.4.1.4 Pseudarthrobacter chlorophenolicus DSM 12829 B8H6N5
-
-
2.4.1.4 Synechococcus sp. SMQ77851
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.4 n sucrose 45% polymerization products, additionally ASase catalyzes reactions with 8% isomerization products and 71% hydrolysis products Deinococcus geothermalis [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose 56.9% polymerization products, additionally ASase catalyzes reactions with 33.5% isomerization products and 9.6% hydrolysis products Deinococcus radiodurans [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose 75.5% polymerization products, additionally ASase catalyzes reactions with 15.0% isomerization products and 9.5% hydrolysis products Methylobacillus flagellatus [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose 78.4% polymerization products, additionally ASase catalyzes reactions with 19.7% isomerization products and 1.9% hydrolysis products Pseudarthrobacter chlorophenolicus [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose 80.1% polymerization products, additionally ASase catalyzes reactions with 14.5% isomerization products and 5.4% hydrolysis products Neisseria polysaccharea [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose 82.7% polymerization products, additionally ASase catalyzes reactions with 11.5% isomerization products and 5.8% hydrolysis products Deinococcus radiopugnans [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose 84% polymerization products, additionally ASase catalyzes reactions with 10.2% isomerization products and 5.8% hydrolysis products Cellulomonas carbonis [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions Methylotuvimicrobium alcaliphilum [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions Neisseria subflava [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions Alteromonas macleodii [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions Synechococcus sp. [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions Neisseria subflava NJ9703 [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose 45% polymerization products, additionally ASase catalyzes reactions with 8% isomerization products and 71% hydrolysis products Deinococcus geothermalis DSM 11300 [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose 84% polymerization products, additionally ASase catalyzes reactions with 10.2% isomerization products and 5.8% hydrolysis products Cellulomonas carbonis T26 [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose additionally to polymerization, ASase catalyzes isomerization and hydrolysis reactions Methylotuvimicrobium alcaliphilum DSM 19304 [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose 56.9% polymerization products, additionally ASase catalyzes reactions with 33.5% isomerization products and 9.6% hydrolysis products Deinococcus radiodurans ATCC 13939 [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?
2.4.1.4 n sucrose 78.4% polymerization products, additionally ASase catalyzes reactions with 19.7% isomerization products and 1.9% hydrolysis products Pseudarthrobacter chlorophenolicus DSM 12829 [(1->4)-alpha-D-glucosyl]n + n fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.4 dimer
-
 Deinococcus geothermalis
2.4.1.4 dimer
-
 Deinococcus radiopugnans
2.4.1.4 dimer
-
 Deinococcus radiodurans
2.4.1.4 dimer
-
 Methylobacillus flagellatus
2.4.1.4 monomer
-
 Neisseria polysaccharea
2.4.1.4 monomer
-
 Methylotuvimicrobium alcaliphilum
2.4.1.4 monomer
-
 Cellulomonas carbonis
2.4.1.4 monomer
-
 Pseudarthrobacter chlorophenolicus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.4 ASASE
-
 Neisseria polysaccharea
2.4.1.4 ASASE
-
 Deinococcus geothermalis
2.4.1.4 ASASE
-
 Methylotuvimicrobium alcaliphilum
2.4.1.4 ASASE
-
 Deinococcus radiopugnans
2.4.1.4 ASASE
-
 Neisseria subflava
2.4.1.4 ASASE
-
 Cellulomonas carbonis
2.4.1.4 ASASE
-
 Pseudarthrobacter chlorophenolicus
2.4.1.4 ASASE
-
 Alteromonas macleodii
2.4.1.4 ASASE
-
 Deinococcus radiodurans
2.4.1.4 ASASE
-
 Methylobacillus flagellatus
2.4.1.4 ASASE
-
 Synechococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.4 30
-
-
 Methylotuvimicrobium alcaliphilum
2.4.1.4 30
-
-
 Synechococcus sp.
2.4.1.4 37
-
-
 Neisseria polysaccharea
2.4.1.4 40 45
-
 Deinococcus radiopugnans
2.4.1.4 40
-
-
 Cellulomonas carbonis
2.4.1.4 45
-
-
 Deinococcus geothermalis
2.4.1.4 45
-
-
 Neisseria subflava
2.4.1.4 45
-
-
 Alteromonas macleodii
2.4.1.4 45
-
-
 Methylobacillus flagellatus
2.4.1.4 50
-
-
 Deinococcus radiodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.4 30
-
 half-life: 1260 min Neisseria polysaccharea
2.4.1.4 30
-
 half-life: 900 min Deinococcus radiodurans
2.4.1.4 40
-
 half-life: 104 min Pseudarthrobacter chlorophenolicus
2.4.1.4 40
-
 half-life: 16620 min Cellulomonas carbonis
2.4.1.4 40
-
 half-life: 23104 min Neisseria subflava
2.4.1.4 40
-
 half-life: 25260 min Deinococcus radiopugnans
2.4.1.4 40
-
 half-life: 708 min Alteromonas macleodii
2.4.1.4 42.6
-
 Tm-value Pseudarthrobacter chlorophenolicus
2.4.1.4 45
-
 half-life: 30 min Alteromonas macleodii
2.4.1.4 45
-
 half-life: 44 min Cellulomonas carbonis
2.4.1.4 45
-
 half-life: 3 min Pseudarthrobacter chlorophenolicus
2.4.1.4 45
-
 half-life: 25 min Neisseria polysaccharea
2.4.1.4 45
-
 half-life: 198 min Deinococcus radiopugnans
2.4.1.4 45
-
 half-life: 546 min Neisseria subflava
2.4.1.4 47.8
-
 Tm-value Cellulomonas carbonis
2.4.1.4 48.1
-
 Tm-value Alteromonas macleodii
2.4.1.4 49.6
-
 Tm-value Neisseria polysaccharea
2.4.1.4 50
-
 half-life: 20 min Methylobacillus flagellatus
2.4.1.4 50
-
 half-life: 1686 min Deinococcus geothermalis
2.4.1.4 50.6
-
 Tm-value Methylobacillus flagellatus
2.4.1.4 50.7
-
 Tm-value Deinococcus radiopugnans
2.4.1.4 55
-
 half-life: 4 min Methylobacillus flagellatus
2.4.1.4 55
-
 half-life: 408 min Deinococcus geothermalis
2.4.1.4 60.8
-
 Tm-value Deinococcus geothermalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.4 6.5 7
-
 Synechococcus sp.
2.4.1.4 7
-
-
 Cellulomonas carbonis
2.4.1.4 8
-
-
 Neisseria polysaccharea
2.4.1.4 8
-
-
 Deinococcus geothermalis
2.4.1.4 8
-
-
 Methylotuvimicrobium alcaliphilum
2.4.1.4 8
-
-
 Deinococcus radiopugnans
2.4.1.4 8
-
-
 Neisseria subflava
2.4.1.4 8
-
-
 Pseudarthrobacter chlorophenolicus
2.4.1.4 8
-
-
 Alteromonas macleodii
2.4.1.4 8
-
-
 Deinococcus radiodurans
2.4.1.4 8.5
-
-
 Methylobacillus flagellatus