EC Number | Cloned (Comment) | Organism |
---|---|---|
5.6.2.3 | overexpression of the helicase in Drosophila melanogaster Schneider S2 cells, the elevated enzyme levels induce structural rearrangements in the mitochondrial genome of Drosophila melanogaster | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.6.2.3 | additional information | overexpression of the enzyme in Schneider S2 cells, elevated levels of mtDNA helicase alleviate pausing of the replication fork and increase the abundance of replication intermediates. The substantial increase in the signal of RIs in the absence of mtDNA copy number changes suggests that overexpression of mtDNA helicase promotes increased initiation without complete replication of mtDNA molecules. Circular molecules bear deletions in mtDNA from helicase overexpressing cells, elevated levels of mtDNA helicase promote rearrangement of the A+T region, mechanism, overview | Drosophila melanogaster |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
5.6.2.3 | mitochondrion | - |
Drosophila melanogaster | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.6.2.3 | Mg2+ | required | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.2.3 | ATP + H2O | Drosophila melanogaster | - |
ADP + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.2.3 | Drosophila melanogaster | Q9VL76 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.6.2.3 | S2 cell | - |
Drosophila melanogaster | - |
5.6.2.3 | SCHNEIDER-2 cell | - |
Drosophila melanogaster | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.2.3 | ATP + H2O | - |
Drosophila melanogaster | ADP + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.6.2.3 | mtDNA helicase | - |
Drosophila melanogaster |
5.6.2.3 | replicative DNA helicase | - |
Drosophila melanogaster |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.6.2.3 | 37 | - |
assay at | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.6.2.3 | 8 | - |
assay at | Drosophila melanogaster |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.6.2.3 | malfunction | mtDNA helicase mutations are associated with structural rearrangements of the mitochondrial genome, elevation of mtDNA helicase levels increases the quantity of replication intermediates and alleviates pausing at the replication slow zones. A concomitant alteration in mtDNA copy number is not observed, but deletions specific to the segment of repeated elements in the immediate vicinity of the origin of replication, and an accumulation of species characteristic of replication fork stalling. Elevated levels of RNA that are retained in the replication intermediates are also found. Effects of elevated levels of the mtDNA helicase on the integrity and replication of the mitochondrial genome, overview | Drosophila melanogaster |
5.6.2.3 | metabolism | pathological conditions impairing functions of mitochondria often lead to compensatory upregulation of the mitochondrial DNA (mtDNA) replisome machinery, and the replicative DNA helicase appears to be a key factor in regulating mtDNA copy number. Upregulation of mtDNA helicase promotes the process of mtDNA replication but also results in genome destabilization | Drosophila melanogaster |
5.6.2.3 | physiological function | the enzyme is involved in the replication of the mitochondrial genome. Replication of mtDNA requires two key mitochondrial enzymes: DNA polymerase gamma (Pol gamma) and mtDNA helicase. Upregulation of mtDNA helicase promotes the process of mtDNA replication but also results in genome destabilization | Drosophila melanogaster |