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Literature summary extracted from

  • Escalera-Fanjul, X.; Campero-Basaldua, C.; Colon, M.; Gonzalez, J.; Marquez, D.; Gonzalez, A.
    Evolutionary diversification of alanine transaminases in yeast Catabolic specialization and biosynthetic redundancy (2017), Front. Microbiol., 8, 1150 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.2 expressed in Escherichia coli Rosetta2 (DE3) cells Kluyveromyces lactis
2.6.1.2 expressed in Escherichia coli Rosetta2 (DE3) cells Lachancea kluyveri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 0.22
-
 2-oxoglutarate pH and temperature not specified in the publication Lachancea kluyveri
2.6.1.2 0.92
-
 2-oxoglutarate pH and temperature not specified in the publication Kluyveromyces lactis
2.6.1.2 4.88
-
 L-alanine pH and temperature not specified in the publication Lachancea kluyveri
2.6.1.2 17.25
-
 L-alanine pH and temperature not specified in the publication Kluyveromyces lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 100100
-
 dynamic light scattering Lachancea kluyveri
2.6.1.2 135900
-
 dynamic light scattering Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.2 L-alanine + 2-oxoglutarate Saccharomyces cerevisiae
-
 pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate Kluyveromyces lactis
-
 pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate Lachancea kluyveri
-
 pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate Kluyveromyces lactis 155-2
-
 pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate Lachancea kluyveri Y156-2
-
 pyruvate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Kluyveromyces lactis
-
-
-
2.6.1.2 Kluyveromyces lactis 155-2
-
-
-
2.6.1.2 Lachancea kluyveri
-
-
-
2.6.1.2 Lachancea kluyveri Y156-2
-
-
-
2.6.1.2 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2 Ni-NTA agarose column chromatography Kluyveromyces lactis
2.6.1.2 Ni-NTA agarose column chromatography Lachancea kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Saccharomyces cerevisiae pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Kluyveromyces lactis pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Lachancea kluyveri pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Kluyveromyces lactis 155-2 pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Lachancea kluyveri Y156-2 pyruvate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.2 homodimer 2 * 50000, SDS-PAGE Kluyveromyces lactis
2.6.1.2 homodimer 2 * 50000, SDS-PAGE Lachancea kluyveri
2.6.1.2 homodimer 2 * 59230, calculated from amino acid sequence Kluyveromyces lactis
2.6.1.2 homodimer 2 * 61160, calculated from amino acid sequence Lachancea kluyveri

Synonyms

EC Number Synonyms Comment Organism
2.6.1.2 ALT1
-
 Saccharomyces cerevisiae
2.6.1.2 ALT1
-
 Kluyveromyces lactis
2.6.1.2 ALT1
-
 Lachancea kluyveri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.2 203
-
 L-alanine pH and temperature not specified in the publication Kluyveromyces lactis
2.6.1.2 355
-
 L-alanine pH and temperature not specified in the publication Lachancea kluyveri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.2 7
-
-
 Kluyveromyces lactis
2.6.1.2 7
-
-
 Lachancea kluyveri

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.2 pyridoxal 5'-phosphate
-
 Saccharomyces cerevisiae
2.6.1.2 pyridoxal 5'-phosphate
-
 Kluyveromyces lactis
2.6.1.2 pyridoxal 5'-phosphate
-
 Lachancea kluyveri