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Literature summary extracted from

  • Zak, K.M.; Kalilska, M.; Wator, E.; Kuska, K.; Krutyholowa, R.; Dubin, G.; Popowicz, G.M.; Grudnik, P.
    Crystal structure of Kluyveromyces lactis glucokinase (KlGlk1) (2019), Int. J. Mol. Sci., 20, 4821 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.2 the KLLA0C01155g gene is codon optimized for improved expression in Escherichia coli. Expression in Escherichia coli BL21 (DE3) Kluyveromyces lactis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.2 the enzyme is crystallized in an apo form. Sitting-drop vapor diffusion at room temperature Kluyveromyces lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.2 ATP the enzyme exists in two states with different catalytic activity, with an apparent transition point at 0.045 mM ATP. At higher ATP concentrations, strong inhibition takes place Kluyveromyces lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.2 0.08
-
 D-glucose pH and temperature not specified in the publication Kluyveromyces lactis
2.7.1.2 0.15
-
 ATP pH and temperature not specified in the publication Kluyveromyces lactis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.2 Kluyveromyces lactis Q6CUZ3
-
-
2.7.1.2 Kluyveromyces lactis ATCC 8585 Q6CUZ3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.2
-
 Kluyveromyces lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.2 ATP + D-glucose no activity with fructose Kluyveromyces lactis ADP + D-glucose 6-phosphate
-
 ?
2.7.1.2 ATP + D-glucose no activity with fructose Kluyveromyces lactis ATCC 8585 ADP + D-glucose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.2 KlGlk1
-
 Kluyveromyces lactis
2.7.1.2 KLLA0C01155g
-
 Kluyveromyces lactis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.2 41.9
-
 unfolding temperature in thermal shift assay Kluyveromyces lactis
2.7.1.2 53.6
-
 unfolding temperature in Tycho analysis Kluyveromyces lactis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.2 150
-
 D-glucose pH and temperature not specified in the publication Kluyveromyces lactis
2.7.1.2 400
-
 ATP pH and temperature not specified in the publication Kluyveromyces lactis

General Information

EC Number General Information Comment Organism
2.7.1.2 metabolism the enzyme is an essential component required for glucose signaling Kluyveromyces lactis