EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.5 | expressed in tobacco protoplasts, Escherichia coli Rosetta (DE3) cells and Escherichia coli tyrosine auxotrophic mutant DL39 | Prunella vulgaris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.5 | 0.4 | - |
L-tyrosine | with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.48 | - |
4-hydroxyphenylpyruvate | with L-glutamate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.84 | - |
phenylpyruvate | with L-glutamate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 1.82 | - |
oxaloacetate | with L-glutamate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 3.9 | - |
phenylpyruvate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 4.72 | - |
oxaloacetate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 8.14 | - |
L-tryptophan | with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 10.2 | - |
L-phenylalanine | with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 10.8 | - |
2-oxoglutarate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 52.06 | - |
pyruvate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.6.1.5 | cytoplasm | - |
Prunella vulgaris | 5737 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.5 | L-tyrosine + 2-oxoglutarate | Prunella vulgaris | - |
4-hydroxyphenylpyruvate + L-glutamate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.5 | Prunella vulgaris | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.6.1.5 | leaf | - |
Prunella vulgaris | - |
2.6.1.5 | root | highest expression | Prunella vulgaris | - |
2.6.1.5 | spike | - |
Prunella vulgaris | - |
2.6.1.5 | stem | - |
Prunella vulgaris | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.5 | L-glutamate + 4-hydroxyphenylpyruvate | - |
Prunella vulgaris | 2-oxoglutarate + L-tyrosine | - |
r | |
2.6.1.5 | L-glutamate + oxaloacetate | - |
Prunella vulgaris | ? | - |
r | |
2.6.1.5 | L-glutamate + phenylpyruvate | - |
Prunella vulgaris | 2-oxoglutarate + L-phenylalanine | - |
r | |
2.6.1.5 | L-phenylalanine + 2-oxoglutarate | - |
Prunella vulgaris | phenylpyruvate + L-glutamate | - |
r | |
2.6.1.5 | L-tryptophan + 2-oxoglutarate | - |
Prunella vulgaris | 3-(1H-indol-3-yl)-2-oxopropanoate + L-glutamate | - |
r | |
2.6.1.5 | L-tyrosine + 2-oxoglutarate | - |
Prunella vulgaris | 4-hydroxyphenylpyruvate + L-glutamate | - |
r | |
2.6.1.5 | L-tyrosine + oxaloacetate | - |
Prunella vulgaris | 4-hydroxyphenylpyruvate + L-aspartate | - |
r | |
2.6.1.5 | L-tyrosine + phenylpyruvate | - |
Prunella vulgaris | 4-hydroxyphenylpyruvate + L-phenylalanine | - |
r | |
2.6.1.5 | L-tyrosine + pyruvate | - |
Prunella vulgaris | 4-hydroxyphenylpyruvate + L-alanine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.5 | ? | x * 45000, SDS-PAGE | Prunella vulgaris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.5 | TAT | - |
Prunella vulgaris |
2.6.1.5 | tyrosine aminotransferase | - |
Prunella vulgaris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.5 | 30 | - |
- |
Prunella vulgaris |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.5 | 0.11 | - |
oxaloacetate | with L-glutamate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.121 | - |
4-hydroxyphenylpyruvate | with L-glutamate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.15 | - |
phenylpyruvate | with L-glutamate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.3 | - |
phenylpyruvate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.63 | - |
2-oxoglutarate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.73 | - |
oxaloacetate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.89 | - |
pyruvate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 1.05 | - |
L-tryptophan | with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 1.19 | - |
L-tyrosine | with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 1.55 | - |
L-phenylalanine | with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.5 | 8 | - |
- |
Prunella vulgaris |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.6.1.5 | Prunella vulgaris | calculated from amino acid sequence | - |
6 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.6.1.5 | metabolism | the enzyme is involved in rosmarinic acid biosynthesis | Prunella vulgaris |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.5 | 0.02 | - |
pyruvate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.06 | - |
oxaloacetate | with L-glutamate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.06 | - |
2-oxoglutarate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.076 | - |
phenylpyruvate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.13 | - |
L-tryptophan | with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.15 | - |
L-phenylalanine | with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.15 | - |
oxaloacetate | with L-tyrosine as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.18 | - |
phenylpyruvate | with L-glutamate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 0.295 | - |
4-hydroxyphenylpyruvate | with L-glutamate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris | |
2.6.1.5 | 3.03 | - |
L-tyrosine | with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C | Prunella vulgaris |