Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ziogiene, D.; Valaviciute, M.; Norkiene, M.; Timinskas, A.; Gedvilaite, A.
    Mutations of Kluyveromyces lactis dolichol kinase enhances secretion of recombinant proteins (2019), FEMS Yeast Res., 19, foz024 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.108 gene SEC59, genotyping, DNA and amino acid sequence determination and analysis Kluyveromyces lactis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.108 G405S naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype Kluyveromyces lactis
2.7.1.108 G405S/I419S site-directed mutagenesis, despite some glycosylation defects, double DK mutations (G405S and I419S) in the Kluyveromyces lactis mutant strain demonstrate three times the level of recombinant alpha-amylase secretion compared to wild-type strain. Overexpression of potential suppressors KlMNN10, KlSEL1, KlERG20, KlSRT1, KlRER2, KlCAX4, KlLPP1 and KlDPP1 in the DK-mutant strain restores carboxypeptidase Y glycosylation to different extents and, with the exception of KISRT1, reduces alpha-amylase secretion to levels observed in wild-type cells Kluyveromyces lactis
2.7.1.108 I419S naturally occuring mutation, identified in the isolated mutant strain MD2/1-9, the mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype Kluyveromyces lactis
2.7.1.108 additional information phenotypic characterization of CBS2359-KlSEC59 mutants, overview Kluyveromyces lactis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.108 Kluyveromyces lactis Q6CR79 i.e. Candida sphaerica
-
2.7.1.108 Kluyveromyces lactis ATCC 8585 Q6CR79 i.e. Candida sphaerica
-
2.7.1.108 Kluyveromyces lactis CBS 2359 Q6CR79 i.e. Candida sphaerica
-
2.7.1.108 Kluyveromyces lactis DSM 70799 Q6CR79 i.e. Candida sphaerica
-
2.7.1.108 Kluyveromyces lactis NBRC 1267 Q6CR79 i.e. Candida sphaerica
-
2.7.1.108 Kluyveromyces lactis NRRL Y-1140 Q6CR79 i.e. Candida sphaerica
-
2.7.1.108 Kluyveromyces lactis WM37 Q6CR79 i.e. Candida sphaerica
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.108 SEC59
-
 Kluyveromyces lactis

General Information

EC Number General Information Comment Organism
2.7.1.108 malfunction two mutations in the Kluyveromyces lactis KlSEC59 gene, encoding dolichol kinase (DK), are responsible for an enhanced secretion phenotype in the previously isolated mutant, MD2/1-9. Compared with the temperature-sensitive Saccharomyces cerevisiae sec59-1 mutant, which exhibits reduced N-glycosylation and decreased secretory efficacy, the identified Kluyveromyces lactis DK mutations has fewer effects on glycosylation, as well as on survival at high temperature and cell wall integrity. Mutation leads to reduced dolichol kinase activity and the enhanced secretion phenotype. Despite some glycosylation defects, double DK mutations (G405S/I419S) in the Kluyveromyces lactis mutant strain demonstrate three times the level of recombinant alpha-amylase secretion compared to wild-type strain. Overexpression of potential suppressors KlMNN10, KlSEL1, KlERG20, KlSRT1, KlRER2, KlCAX4, KlLPP1 and KlDPP1 in the DK-mutant strain restores carboxypeptidase Y glycosylation to different extents and, with the exception of KISRT1, reduces alpha-amylase secretion to levels observed in wild-type cells. The mutant strain shows substantial reduction of protein N-glycosylation, secretion efficacy, and growth rate, particularly at the nonpermissive temperature Kluyveromyces lactis