Literature summary extracted from

Liu, J.J.; Zhang, G.C.; Kwak, S.; Oh, E.J.; Yun, E.J.; Chomvong, K.; Cate, J.H.D.; Jin, Y.S.
Overcoming the thermodynamic equilibrium of an isomerization reaction through oxidoreductive reactions for biotransformation (2019), Nat. Commun., 10, 1356 .

Application

EC Number	Application	Comment	Organism
1.1.1.16	synthesis	a yeast strain capable of consuming lactose intracellularly is engineered to produce tagatose from lactose. GAL1 coding for galactose kinase is deleted to eliminate galactose utilization. Heterologous xylose reductase (XR) and galactitol dehydrogenase (GDH) are introduced into the Gal1 deletion strain. The expression levels of XR and GDH are adjusted to maximize tagatose production. The resulting engineered yeast produces 37.69 g/l of tagatose from lactose with a tagatose and galactose ratio of 9:1 in the reaction broth	Rhizobium leguminosarum bv. viciae
1.1.1.406	synthesis	the two oxidoreductases, xylose reductase and galactitol dehydrogenase are functionally expressed in the engineered yeast (EJ2g_pXpG) and enable direct production of tagatose from lactose. The expression levels of the enzymes are adjusted to maximize tagatose production. The resulting engineered yeast produces 37.69 g/L of tagatose from lactose with a tagatose and galactose ratio of 9:1 in the reaction broth	Rhizobium leguminosarum

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.16	expression in Saccharomyces cerevisiae	Rhizobium leguminosarum bv. viciae
1.1.1.406	expressed in the engineered yeast (EJ2g_pXpG)	Rhizobium leguminosarum

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.16	Rhizobium leguminosarum bv. viciae	Q1MLL4	-	-
1.1.1.406	Rhizobium leguminosarum	-	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.16	GDH	-	Rhizobium leguminosarum bv. viciae
1.1.1.406	galactitol dehydrogenase	-	Rhizobium leguminosarum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)