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Literature summary extracted from

  • Li, M.; Zhu, W.; Meng, Q.; Miao, M.; Zhang, T.
    Characterization of xylitol 4-dehydrogenase from Erwinia aphidicola and its co-expression with NADH oxidase in Bacillus subtilis (2021), Process Biochem., 104, 92-100 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.B19 synthesis enzyme XDH from Erwinia aphidicola can be useful for production of L-xylulose, a rare ketose, for apllication in pharmaceutical and food industries Erwinia aphidicola

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B19 recombinant expression in Bacillus subtilis, coexpression with the water-forming enzyme NADH oxidase (NOX) for NAD+ recycling leading to 3.4fold increased XDH activity Erwinia aphidicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B19 18.71
-
 xylitol recombinant enzyme, pH 11.0, 45°C Erwinia aphidicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B19 xylitol + NAD+ Erwinia aphidicola
-
 L-xylulose + NADH + H+
-
 ?
1.1.1.B19 xylitol + NAD+ Erwinia aphidicola SK47.001
-
 L-xylulose + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B19 Erwinia aphidicola
-
-
-
1.1.1.B19 Erwinia aphidicola SK47.001
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B19 recombinant enzyme from Bacillus subtilis Erwinia aphidicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B19 xylitol + NAD+
-
 Erwinia aphidicola L-xylulose + NADH + H+
-
 ?
1.1.1.B19 xylitol + NAD+
-
 Erwinia aphidicola SK47.001 L-xylulose + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B19 XDH
-
 Erwinia aphidicola
1.1.1.B19 xylitol 4-dehydrogenase
-
 Erwinia aphidicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B19 45
-
 recombinant enzyme Erwinia aphidicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B19 11
-
 recombinant enzyme Erwinia aphidicola

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B19 NAD+
-
 Erwinia aphidicola

General Information

EC Number General Information Comment Organism
1.1.1.B19 additional information enzyme structure homology modelling and molecular docking, active site structure analysis, the potential catalytic sites are Ser149, Tyr162, and Lys166 for xylitol and NAD+ Erwinia aphidicola