Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Artiukhov, A.; Kazantsev, A.; Lukashev, N.; Bellinzoni, M.; Bunik, V.
    Selective inhibition of 2-oxoglutarate and 2-oxoadipate dehydrogenases by the phosphonate analogs of their 2-oxo acid substrates (2021), Front. Chem., 8, 596187 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.4.2 adipoyl phosphonate
-
 Rattus norvegicus
1.2.4.2 glutaryl phosphonate
-
 Rattus norvegicus
1.2.4.2 succinyl phosphonate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.4.2 0.008
-
 2-oxoadipate preparation from liver, low affinity state, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.27
-
 2-oxoglutarate enzyme from liver, at pH 7.0, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.27
-
 2-oxoadipate preparation from liver, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.45
-
 2-oxoglutarate enzyme from heart, at pH 7.0, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.45
-
 2-oxoadipate preparation from heart, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.45
-
 2-oxoadipate preparation from liver, low affinity state, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.55
-
 2-oxoadipate preparation from heart, pH 7, temperature not specified in the publication Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.4.2 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.4.2 Ca2+ 1 mM used in assay conditions Rattus norvegicus
1.2.4.2 Mg2+ 1 mM used in assay conditions Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.4.2 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine Rattus norvegicus
-
 [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.2 Rattus norvegicus Q4KLP0
-
-
1.2.4.2 Rattus norvegicus Q5XI78
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.4.2 heart
-
 Rattus norvegicus
-
1.2.4.2 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.2 2-oxoadipate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine
-
 Rattus norvegicus [dihydrolipoyllysine-residue succinyltransferase] S-glutaryldihydrolipoyllysine + CO2
-
 ?
1.2.4.2 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine
-
 Rattus norvegicus [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.4.2 2-oxoadipate dehydrogenase
-
 Rattus norvegicus
1.2.4.2 2-oxoglutarate dehydrogenase
-
 Rattus norvegicus
1.2.4.2 DHTKD1
-
 Rattus norvegicus
1.2.4.2 E1o
-
 Rattus norvegicus
1.2.4.2 OADH
-
 Rattus norvegicus
1.2.4.2 OGDH
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.2 thiamine diphosphate
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.4.2 0.00005
-
 adipoyl phosphonate and 0.50, preparation from liver, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.0014
-
 glutaryl phosphonate and 0.0024, preparation from liver, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.0024
-
 glutaryl phosphonate and 0.0014, preparation from liver, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.0038
-
 succinyl phosphonate and 0.128, preparation from liver, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.0038
-
 glutaryl phosphonate preparation from heart, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.0073
-
 glutaryl phosphonate enzyme from liver, at pH 7.0, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.0096
-
 succinyl phosphonate enzyme from liver, at pH 7.0, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.0096
-
 succinyl phosphonate preparation from liver, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.0102
-
 succinyl phosphonate preparation from heart, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.02
-
 succinyl phosphonate enzyme from heart, at pH 7.0, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.02
-
 succinyl phosphonate preparation from heart, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.039
-
 glutaryl phosphonate enzyme from heart, at pH 7.0, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.039
-
 glutaryl phosphonate preparation from heart, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.049
-
 adipoyl phosphonate preparation from heart, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.05
-
 adipoyl phosphonate and 0.000050, preparation from liver, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.073
-
 glutaryl phosphonate preparation from liver, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.128
-
 succinyl phosphonate and 0.0038, preparation from liver, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.405
-
 adipoyl phosphonate enzyme from liver, at pH 7.0, temperature not specified in the publication Rattus norvegicus
1.2.4.2 0.405
-
 adipoyl phosphonate preparation from liver, pH 7, temperature not specified in the publication Rattus norvegicus
1.2.4.2 2.487
-
 adipoyl phosphonate enzyme from heart, at pH 7.0, temperature not specified in the publication Rattus norvegicus
1.2.4.2 2.487
-
 adipoyl phosphonate preparation from heart, pH 7, temperature not specified in the publication Rattus norvegicus

General Information

EC Number General Information Comment Organism
1.2.4.2 physiological function isoenzymes 2-oxoglutarate dehydrogenase (OGDH) and 2-oxoadipate dehydrogenase (OADH) show a 100fold difference in their ratio in the heart and liver, but similar Michaelis saturations by 2-oxoglutarate are inherent in the enzyme preparations from these tissues. In the heart, OADH/OGDH ratio is about 0.01, and OADH possesses low-affinity sites to 2-oxoadipate. In liver preparation, OADH/OGDH ratio is about 1.6, and OADH a biphasic saturation with 2-oxoadipate Rattus norvegicus