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Literature summary extracted from

  • Yahyaa, M.; Berim, A.; Nawade, B.; Ibdah, M.; Dudareva, N.; Ibdah, M.
    Biosynthesis of methyleugenol and methylisoeugenol in Daucus carota leaves characterization of eugenol/isoeugenol synthase and O-methyltransferase (2019), Phytochemistry, 159, 179-189 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.318 gene DcEGS1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli Daucus carota subsp. sativus
1.1.1.319 gene DcEIGS1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli Daucus carota subsp. sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.319 0.121
-
 NADPH recombinant enzyme, pH 7.0, 30°C Daucus carota subsp. sativus
1.1.1.319 0.248
-
 coniferyl acetate recombinant enzyme, pH 7.0, 30°C Daucus carota subsp. sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.318 coniferyl acetate + NADPH + H+ Daucus carota subsp. sativus
-
 eugenol + acetate + NADP+
-
 r
1.1.1.319 coniferyl acetate + NADPH + H+ Daucus carota subsp. sativus
-
 isoeugenol + acetate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.318 Daucus carota subsp. sativus A0A166G7A0 orange genotype
-
1.1.1.319 Daucus carota subsp. sativus A0A166G7A0 orange genotype
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.318 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Daucus carota subsp. sativus
1.1.1.319 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Daucus carota subsp. sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.318 leaf young and mature leaves Daucus carota subsp. sativus
-
1.1.1.318 additional information quantitative RT-PCR enzyme expression analysis in different tissues. Distribution of aromatic volatiles in different parts of the carrot plants, overview Daucus carota subsp. sativus
-
1.1.1.318 root
-
 Daucus carota subsp. sativus
-
1.1.1.318 seed
-
 Daucus carota subsp. sativus
-
1.1.1.319 leaf young and mature leaves Daucus carota subsp. sativus
-
1.1.1.319 additional information quantitative RT-PCR enzyme expression analysis in different tissues. Distribution of aromatic volatiles in different parts of the carrot plants, overview Daucus carota subsp. sativus
-
1.1.1.319 root
-
 Daucus carota subsp. sativus
-
1.1.1.319 seed
-
 Daucus carota subsp. sativus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.318 coniferyl acetate + NADPH + H+
-
 Daucus carota subsp. sativus eugenol + acetate + NADP+
-
 r
1.1.1.318 additional information purified recombinant DcE(I)GS1 catalyzes the formation of eugenol (70% of total products, EC 1.1.1.318), as well as isoeugenol (30% of total products,, EC 1.1.1.319) from coniferyl acetate Daucus carota subsp. sativus ?
-
-
1.1.1.319 coniferyl acetate + NADPH + H+
-
 Daucus carota subsp. sativus isoeugenol + acetate + NADP+
-
 r
1.1.1.319 additional information purified recombinant DcE(I)GS1 catalyzes the formation of eugenol (70% of total products, EC 1.1.1.318), as well as isoeugenol (30% of total products,, EC 1.1.1.319) from coniferyl acetate Daucus carota subsp. sativus ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.318 DcE(I)GS1
-
 Daucus carota subsp. sativus
1.1.1.318 DcEGS1
-
 Daucus carota subsp. sativus
1.1.1.318 eugenol/isoeugenol synthase
-
 Daucus carota subsp. sativus
1.1.1.318 More see also EC 1.1.1.319 Daucus carota subsp. sativus
1.1.1.319 DcE(I)GS1
-
 Daucus carota subsp. sativus
1.1.1.319 DcIGS1
-
 Daucus carota subsp. sativus
1.1.1.319 eugenol/isoeugenol synthase
-
 Daucus carota subsp. sativus
1.1.1.319 More see also EC 1.1.1.318 Daucus carota subsp. sativus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.318 30
-
 recombinant His-tagged enzyme Daucus carota subsp. sativus
1.1.1.319 30
-
 recombinant His-tagged enzyme Daucus carota subsp. sativus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.319 0.46
-
 coniferyl acetate recombinant enzyme, pH 7.0, 30°C Daucus carota subsp. sativus
1.1.1.319 0.68
-
 NADPH recombinant enzyme, pH 7.0, 30°C Daucus carota subsp. sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.318 7
-
 recombinant His-tagged enzyme Daucus carota subsp. sativus
1.1.1.319 7
-
 recombinant His-tagged enzyme Daucus carota subsp. sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.318 4 8 activity range Daucus carota subsp. sativus
1.1.1.319 4 8 activity range Daucus carota subsp. sativus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.318 NADP+
-
 Daucus carota subsp. sativus
1.1.1.318 NADPH
-
 Daucus carota subsp. sativus
1.1.1.319 NADP+
-
 Daucus carota subsp. sativus
1.1.1.319 NADPH
-
 Daucus carota subsp. sativus

General Information

EC Number General Information Comment Organism
1.1.1.318 additional information distribution of aromatic volatiles in different parts of the carrot plants, overview Daucus carota subsp. sativus
1.1.1.318 physiological function eugenol/isoeugenol synthase (DcE(I)GS1) is an NADPH-dependent enzyme that converts coniferyl acetate to eugenol. This enzyme exhibits dual product specificity and yields propenylphenol isoeugenol alongside allylphenol eugenol Daucus carota subsp. sativus
1.1.1.319 additional information distribution of aromatic volatiles in different parts of the carrot plants, overview Daucus carota subsp. sativus
1.1.1.319 physiological function eugenol/isoeugenol synthase (DcE(I)GS1) is an NADPH-dependent enzyme that converts coniferyl acetate to eugenol. This enzyme exhibits dual product specificity and yields propenylphenol isoeugenol alongside allylphenol eugenol Daucus carota subsp. sativus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.319 1.855
-
 coniferyl acetate recombinant enzyme, pH 7.0, 30°C Daucus carota subsp. sativus
1.1.1.319 5.62
-
 NADPH recombinant enzyme, pH 7.0, 30°C Daucus carota subsp. sativus