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Literature summary extracted from
- Crystal structure of heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96 (2005), Biochem. Biophys. Res. Commun., 333, 359-366 .
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.5.3.1 | enzyme in complex with dimethylglycine and folinic acid, vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 1.9 M ammonium sulfate, and 10 mM CuSO4 | Corynebacterium sp. U-96 |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.3.1 | Zn2+ | the delta subunit contains a single atom of zinc | Corynebacterium sp. U-96 |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.3.1 | sarcosine + 5,6,7,8-tetrahydrofolate + O2 | Corynebacterium sp. U-96 | - |
glycine + formaldehyde + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.3.1 | Corynebacterium sp. U-96 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.3.1 | HiTrap DEAE column chromatography, HisTrap column chromatography, HiTrap phenyl column chromatography, Resources Q column chromatography, and Superdex 200 gel filtration | Corynebacterium sp. U-96 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.3.1 | sarcosine + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. U-96 | glycine + formaldehyde + H2O2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.3.1 | heterotetramer | x-ray crystallography | Corynebacterium sp. U-96 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.3.1 | heterotetrameric sarcosine oxidase | - |
Corynebacterium sp. U-96 |
| 1.5.3.1 | TSOX | - |
Corynebacterium sp. U-96 |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.3.1 | FAD | the beta subunit contains FAD | Corynebacterium sp. U-96 | |
| 1.5.3.1 | FMN | the beta subunit contains FMN | Corynebacterium sp. U-96 | |
| 1.5.3.1 | NAD+ | the alpha subunit contains NAD+ | Corynebacterium sp. U-96 | |
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