Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Seo, M.J.; Kang, W.R.; Yang, E.J.; Shin, K.C.; Ko, Y.J.; Oh, D.K.
    Molecular characterization of Penicillium oxalicum 6R,8R-linoleate diol synthase with new regiospecificity (2019), Biochim. Biophys. Acta Mol. Cell Biol. Lipids, 1864, 577-586 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.60 expressed in Escherichia ER2566 cells Penicillium oxalicum

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.60 C1005S the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation Penicillium oxalicum
1.13.11.60 D749A the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 E728A the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 F794E the mutant acts as 7,8-linoleate diol synthase Penicillium oxalicum
1.13.11.60 F794Y the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 F798Y the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 H1003A the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 I841A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 L970A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 M1051A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 M695A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 M883A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 N886D the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation Penicillium oxalicum
1.13.11.60 P1016A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 Q887A the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 Q889A the mutant acts as 8,11-linoleate diol synthase Penicillium oxalicum
1.13.11.60 Q889A the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation Penicillium oxalicum
1.13.11.60 R707A the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation Penicillium oxalicum
1.13.11.60 R814A the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 R934A the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation Penicillium oxalicum
1.13.11.60 T879A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 V1010A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 V884A the mutant shows more than 110% activity of the wild type enzyme Penicillium oxalicum
1.13.11.60 Y690F the mutant shows less than 10% activity of the wild type enzyme Penicillium oxalicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.60 0.0264
-
 linoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
1.13.11.60 0.0283
-
 oleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
1.13.11.60 0.0329
-
 alpha-linolenic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
1.13.11.60 0.0475
-
 palmitoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.60 370000
-
 gel filtration Penicillium oxalicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.60 linoleate + O2 Penicillium oxalicum
-
 (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate
-
 ?
1.13.11.60 linoleate + O2 Penicillium oxalicum KCTC 6440
-
 (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.60 Penicillium oxalicum
-
-
-
1.13.11.60 Penicillium oxalicum KCTC 6440
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.60 His-Trap column chromatography Penicillium oxalicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.60 alpha-linolenic acid + O2
-
 Penicillium oxalicum (6S,8R,9Z,12Z,15Z)-dihydroxy-9,12,15-octadecatrienoic acid
-
 ?
1.13.11.60 alpha-linolenic acid + O2
-
 Penicillium oxalicum KCTC 6440 (6S,8R,9Z,12Z,15Z)-dihydroxy-9,12,15-octadecatrienoic acid
-
 ?
1.13.11.60 linoleate + O2
-
 Penicillium oxalicum (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate
-
 ?
1.13.11.60 linoleate + O2
-
 Penicillium oxalicum KCTC 6440 (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate
-
 ?
1.13.11.60 linoleic acid + O2 highest activity Penicillium oxalicum (6R,8R)-dihydroxy-9,12(Z,Z)-octadecadienoic acid
-
 ?
1.13.11.60 linoleic acid + O2 highest activity Penicillium oxalicum KCTC 6440 (6R,8R)-dihydroxy-9,12(Z,Z)-octadecadienoic acid
-
 ?
1.13.11.60 oleic acid + O2
-
 Penicillium oxalicum (6R,8R,9Z)-dihydroxy-9-octadecenoic acid
-
 ?
1.13.11.60 oleic acid + O2
-
 Penicillium oxalicum KCTC 6440 (6R,8R,9Z)-dihydroxy-9-octadecenoic acid
-
 ?
1.13.11.60 palmitoleic acid + O2
-
 Penicillium oxalicum (6S,8R,9Z)-dihydroxy-9-hexadecenoic acid
-
 ?
1.13.11.60 palmitoleic acid + O2
-
 Penicillium oxalicum KCTC 6440 (6S,8R,9Z)-dihydroxy-9-hexadecenoic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.60 homotrimer 3 * 121000, full-length enzyme, SDS-PAGE Penicillium oxalicum

Synonyms

EC Number Synonyms Comment Organism
1.13.11.60 6,8-diol synthase
-
 Penicillium oxalicum
1.13.11.60 6R,8R-linoleate diol synthase
-
 Penicillium oxalicum
1.13.11.60 6R,8RLDS
-
 Penicillium oxalicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.60 25
-
-
 Penicillium oxalicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.60 50
-
 the enzyme exhibits 28% of its initial activity at 50°C after 2 h Penicillium oxalicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.60 4.34
-
 alpha-linolenic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
1.13.11.60 21.4
-
 palmitoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
1.13.11.60 30.4
-
 oleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
1.13.11.60 32.8
-
 linoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.60 6.5
-
-
 Penicillium oxalicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.60 130
-
 alpha-linolenic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
1.13.11.60 450
-
 palmitoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
1.13.11.60 1070
-
 oleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum
1.13.11.60 1240
-
 linoleic acid full-length enzyme, at pH 6.5 and 25°C Penicillium oxalicum