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Literature summary extracted from

  • Saito, M.; Kanno, M.; Iizuka, H.; Suzuki, H.
    Kinetic studies on the role of Lys-171 and Lys-358 in the beta subunit of sarcosine oxidase from Corynebacterium sp. U-96 (2007), J. Biochem., 141, 799-815 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.24 expressed in Escherichia coli BL21(DE3) cells Corynebacterium sp. U-96

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.24 K171A the mutant shows 39% activity of the wild type enzyme Corynebacterium sp. U-96
1.5.3.24 K171D the mutant shows 32% activity of the wild type enzyme Corynebacterium sp. U-96
1.5.3.24 K171R the mutant shows 58% activity of the wild type enzyme Corynebacterium sp. U-96
1.5.3.24 K358A inactive Corynebacterium sp. U-96
1.5.3.24 K358D inactive Corynebacterium sp. U-96
1.5.3.24 K358R the mutant shows 0.07% activity and higher apparent Km for sarcosine than the wild type enzyme Corynebacterium sp. U-96

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.24 0.0312
-
 O2 mutant enzyme K171R, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 0.0329
-
 O2 wild type enzyme, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 0.386
-
 O2 mutant enzyme K171A, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 0.73
-
 O2 mutant enzyme K171D, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 1.47
-
 sarcosine mutant enzyme K171A, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 1.72
-
 sarcosine mutant enzyme K171R, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 2.75
-
 sarcosine mutant enzyme K171D, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 2.76
-
 sarcosine wild type enzyme, at pH 8.0 and 25°C Corynebacterium sp. U-96
1.5.3.24 17.4
-
 sarcosine mutant enzyme K358R, at pH 8.0 and 25°C Corynebacterium sp. U-96

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.24 sarcosine + 5,6,7,8-tetrahydrofolate + O2 Corynebacterium sp. U-96
-
 glycine + 5,10-methylenetetrahydrofolate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.24 Corynebacterium sp. U-96
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.24 sarcosine + 5,6,7,8-tetrahydrofolate + O2
-
 Corynebacterium sp. U-96 glycine + 5,10-methylenetetrahydrofolate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.24 heterotetrameric sarcosine oxidase
-
 Corynebacterium sp. U-96

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.24 FAD
-
 Corynebacterium sp. U-96
1.5.3.24 FMN
-
 Corynebacterium sp. U-96