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Literature summary extracted from
- Preparation, reconstruction, and characterization of a predicted Thermomicrobium roseum sarcosine oxidase (2018), Mol. Catal., 455, 132-142 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.3.1 | expression in Escherichia coli as inclusion body | Thermomicrobium roseum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.5.3.1 | F235V/F339L | mutant can be expressed as soluble protein | Thermomicrobium roseum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.3.1 | 19.7 | - |
sarcosine | wild-type, reconstituted in presence of 7-chloro-8-ethyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum |  |
| 1.5.3.1 | 47.2 | - |
sarcosine | wild-type, reconstituted in presence of 3-methyl-7,8-dimethyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 81.9 | - |
sarcosine | wild-type, reconstituted in presence of FMN, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 85.9 | - |
sarcosine | wild-type, reconstituted in presence of 7-methyl-8-bromo-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 112.5 | - |
sarcosine | wild-type, reconstituted in presence of 7-methyl-8-chloro-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 128.9 | - |
sarcosine | mutant F235V/F339L, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 134.7 | - |
sarcosine | wild-type, reconstituted in presence of 7,8-diethyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 137.2 | - |
sarcosine | wild-type, reconstituted in presence of FAD, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 153.6 | - |
sarcosine | wild-type, reconstituted in presence of isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 183.1 | - |
sarcosine | wild-type, reconstituted in presence of 7-bromo-8-methyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.3.1 | Thermomicrobium roseum | B9L163 | - |
- |
| 1.5.3.1 | Thermomicrobium roseum DSM 5159 | B9L163 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.3.1 | recombinant protein, purified by one step of affinity purification on a Ni-Sepharose column, and another step of ion-exchange purification on a DEAE column | Thermomicrobium roseum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.3.1 | N-methyl-L-tryptophan + O2 + H2O | - |
Thermomicrobium roseum | formaldehyde + L-tryptophan + H2O2 | - |
? | |
| 1.5.3.1 | N-methyl-L-tryptophan + O2 + H2O | - |
Thermomicrobium roseum DSM 5159 | formaldehyde + L-tryptophan + H2O2 | - |
? | |
| 1.5.3.1 | sarcosine + H2O + O2 | - |
Thermomicrobium roseum | glycine + formaldehyde + H2O2 | - |
? | |
| 1.5.3.1 | sarcosine + H2O + O2 | - |
Thermomicrobium roseum DSM 5159 | glycine + formaldehyde + H2O2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.3.1 | ? | x * 43000, SDS-PAGE, recombinant protein | Thermomicrobium roseum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.3.1 | SOX | - |
Thermomicrobium roseum |
| 1.5.3.1 | trd_1773 | - |
Thermomicrobium roseum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.3.1 | 65 | 70 | mutant F235V/F339L | Thermomicrobium roseum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.3.1 | 0.001 | - |
sarcosine | mutant F235V/F339L, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.001 | - |
sarcosine | wild-type, reconstituted in presence of 7,8-diethyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.001 | - |
sarcosine | wild-type, reconstituted in presence of 7-methyl-8-bromo-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.001 | - |
sarcosine | wild-type, reconstituted in presence of FAD, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0011 | - |
sarcosine | wild-type, reconstituted in presence of 7-methyl-8-chloro-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0011 | - |
sarcosine | wild-type, reconstituted in presence of isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0013 | - |
sarcosine | wild-type, reconstituted in presence of 7-bromo-8-methyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0013 | - |
sarcosine | wild-type, reconstituted in presence of 7-chloro-8-ethyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0013 | - |
sarcosine | wild-type, reconstituted in presence of FMN, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0015 | - |
sarcosine | wild-type, reconstituted in presence of 3-methyl-7,8-dimethyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.3.1 | FAD | binds both FAD and FMN. Residue Phe 339 is the cofactor binding site | Thermomicrobium roseum | |
| 1.5.3.1 | FMN | binds both FAD and FMN. Residue Phe 339 is the cofactor binding site | Thermomicrobium roseum | |
| 1.5.3.1 | additional information | reconstructed SOX with a ligand modified at the 7- or 8-position, i.e. by a halogen atom, shows significantly different Km, kcat, relative specificity, optimal reaction temperature, and pH adaptability values compared with refolded SOX-FAD | Thermomicrobium roseum |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.3.1 | 0.000007 | - |
sarcosine | wild-type, reconstituted in presence of 7-bromo-8-methyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0000073 | - |
sarcosine | wild-type, reconstituted in presence of FAD, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0000073 | - |
sarcosine | wild-type, reconstituted in presence of isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0000075 | - |
sarcosine | wild-type, reconstituted in presence of 7,8-diethyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0000078 | - |
sarcosine | mutant F235V/F339L, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0000097 | - |
sarcosine | wild-type, reconstituted in presence of 7-methyl-8-chloro-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0000112 | - |
sarcosine | wild-type, reconstituted in presence of 7-methyl-8-bromo-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0000157 | - |
sarcosine | wild-type, reconstituted in presence of FMN, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0000307 | - |
sarcosine | wild-type, reconstituted in presence of 3-methyl-7,8-dimethyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
| 1.5.3.1 | 0.0000683 | - |
sarcosine | wild-type, reconstituted in presence of 7-chloro-8-ethyl-10-(1'-D-ribityl) isoalloxazine, pH not specified in the publication, 37°C | Thermomicrobium roseum | |
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