Any feedback?
Literature summary extracted from
- The molecular and functional characterization of the durum wheat lipoxygenase TdLOX2 suggests its role in hyperosmotic stress response (2020), Plants (Basel), 9, 1233 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.12 | expressed in Escherichia coli BL21-AI (DE3) cells | Triticum turgidum subsp. durum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.12 | propyl gallate | almost complete inhibition (99%) in the presence of 1 mM propyl gallate | Triticum turgidum subsp. durum |  |
| 1.13.11.12 | Salicylhydroxamic acid | 70% inhibition in the presence of 1 mM salicylhydroxamic acid | Triticum turgidum subsp. durum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.12 | 0.07369 | - |
linoleic acid | at pH 7.5 and 25°C | Triticum turgidum subsp. durum | |
| 1.13.11.12 | 0.3001 | - |
linolenic acid | at pH 7.5 and 25°C | Triticum turgidum subsp. durum | |
| 1.13.11.12 | 0.741 | - |
arachidonic acid | at pH 7.5 and 25°C | Triticum turgidum subsp. durum | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.12 | Triticum turgidum subsp. durum | A0A7M3TWE5 | durum wheat | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.12 | Ni-NTA column chromatography | Triticum turgidum subsp. durum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.13.11.12 | seedling | - |
Triticum turgidum subsp. durum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.12 | arachidonic acid + O2 | very low activity and specific activity towards arachidonic acid | Triticum turgidum subsp. durum | ? | - |
? | |
| 1.13.11.12 | linoleic acid + O2 | preferred substrate | Triticum turgidum subsp. durum | (10E,12Z)-13-hydroperoxy-10,12-octadecadienoate | - |
? | |
| 1.13.11.12 | linolenic acid + O2 | good preference for linolenic acid | Triticum turgidum subsp. durum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.12 | ? | x * 96750, calculated from amino acid sequence | Triticum turgidum subsp. durum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.12 | 13-LOX | - |
Triticum turgidum subsp. durum |
| 1.13.11.12 | LOX2 | - |
Triticum turgidum subsp. durum |
| 1.13.11.12 | Lpx-A2 | - |
Triticum turgidum subsp. durum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 7.5 | - |
- |
Triticum turgidum subsp. durum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.13.11.12 | Triticum turgidum subsp. durum | calculated from amino acid sequence | - |
6.1 |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.13.11.12 | Triticum turgidum subsp. durum | the up-regulation of the enzyme gene is a component of the durum wheat response to hyperosmotic stress. Under mannitol and NaCl stress, a strong increase in the enzyme activity is observed | up |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
