Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Qian, H.; Xia, B.; He, Y.; Lu, Z.; Bie, X.; Zhao, H.; Zhang, C.; Lu, F.
    Expression, purification, and characterization of a novel acidic lipoxygenase from Myxococcus xanthus (2017), Protein Expr. Purif., 138, 13-17 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.11.12 additional information the enzyme can be used for decolorization purposes in pulp, textile, and wastewater treatment industries Myxococcus xanthus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.12 expressed in Escherichia coli BL21(DE3) cells Myxococcus xanthus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.12 Fe3+ complete inhibition at 10 mM Myxococcus xanthus
1.13.11.12 K+ 38% residual activity at 50 mM Myxococcus xanthus
1.13.11.12 Zn2+ 53% residual activity at 50 mM Myxococcus xanthus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.12 0.048
-
 linoleic acid at pH 3.0 and 30°C Myxococcus xanthus
1.13.11.12 0.262
-
 arachidonic acid at pH 3.0 and 30°C Myxococcus xanthus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.12 Al3+ 129% activity at 10 mM Myxococcus xanthus
1.13.11.12 Ca2+ 159% activity at 10 mM Myxococcus xanthus
1.13.11.12 Fe2+ 159% activity at 10 mM Myxococcus xanthus
1.13.11.12 Mn2+ 118% activity at 10 mM Myxococcus xanthus
1.13.11.12 Na+ 158% activity at 50 mM Myxococcus xanthus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.12 Myxococcus xanthus Q1DBH9
-
-
1.13.11.12 Myxococcus xanthus DK1622 Q1DBH9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.12 Ni-NTA column chromatography Myxococcus xanthus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.12 aniline blue + O2 69.7% decolorization after 35 min incubation Myxococcus xanthus ?
-
 ?
1.13.11.12 aniline blue + O2 69.7% decolorization after 35 min incubation Myxococcus xanthus DK1622 ?
-
 ?
1.13.11.12 arachidonic acid + O2
-
 Myxococcus xanthus ?
-
 ?
1.13.11.12 arachidonic acid + O2
-
 Myxococcus xanthus DK1622 ?
-
 ?
1.13.11.12 linoleic acid + O2 best substrate Myxococcus xanthus (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?
1.13.11.12 linoleic acid + O2 best substrate Myxococcus xanthus DK1622 (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.12 ? x * 80000, SDS-PAGE Myxococcus xanthus

Synonyms

EC Number Synonyms Comment Organism
1.13.11.12 acidic lipoxygenase
-
 Myxococcus xanthus
1.13.11.12 LOX
-
 Myxococcus xanthus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.12 30
-
 enzyme activity gradually decreases at temperatures above 30°C Myxococcus xanthus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.12 20 40 the relative activity is greater than 60% within the temperature range of 20-40°C Myxococcus xanthus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.12 25 60 the thermal stability is highest at 25°C. Less than 50% of the original activity remains after heat treatment at 50°C for 5 min, and the enzyme is completely inactive after treatment at 60°C for 10 min Myxococcus xanthus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.12 13.3
-
 linoleic acid at pH 3.0 and 30°C Myxococcus xanthus
1.13.11.12 66
-
 arachidonic acid at pH 3.0 and 30°C Myxococcus xanthus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.12 3
-
-
 Myxococcus xanthus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.12 2.5 3.5 about 85% activity at pH 2.5, 100% activity at pH 3.0, about 85% activity at pH 3.5. The enzyme activity decreases dramatically at pH values greater than 4.0, while catalytic activity is barely detected at pH values of greater than 6.0 Myxococcus xanthus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.12 6
-
 the enzyme retains about 95% activity after 10 min at pH 6.0. After 10 min incubation at pH 3.0-5.0 or 7.0-10.0, the enzyme retains about 60-70% of the original activity Myxococcus xanthus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.12 251.9
-
 arachidonic acid at pH 3.0 and 30°C Myxococcus xanthus
1.13.11.12 277.1
-
 linoleic acid at pH 3.0 and 30°C Myxococcus xanthus