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Literature summary extracted from
- Pyroglutamate-peptidase from Bacillus amyloliquefaciens. An improved purification method and some properties of the enzyme (1984), Biochim. Biophys. Acta, 791, 117-122.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.3 | 5,5'-dithiobis(2-nitrobenzoic acid) | activity is restored by 2-mercaptoethanol | Bacillus amyloliquefaciens |  |
| 3.4.19.3 | Nalpha-benzyloxycarbonyl-L-pyroglutamyl chloromethylketone | - |
Bacillus amyloliquefaciens | |
| 3.4.19.3 | PCMB | - |
Bacillus amyloliquefaciens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.3 | 0.49 | - |
luteinizing-hormone-releasing hormone | - |
Bacillus amyloliquefaciens | |
| 3.4.19.3 | 0.59 | - |
thyroliberin-releasing hormone | - |
Bacillus amyloliquefaciens | |
| 3.4.19.3 | 1.91 | - |
neurotensin | - |
Bacillus amyloliquefaciens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | 24000 | - |
3 * 24000, SDS-PAGE | Bacillus amyloliquefaciens |
| 3.4.19.3 | 72000 | - |
gel filtration | Bacillus amyloliquefaciens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.3 | Bacillus amyloliquefaciens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.3 | - |
Bacillus amyloliquefaciens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | additional information | - |
- |
Bacillus amyloliquefaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.3 | luteinizing-hormone-releasing hormone + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
| 3.4.19.3 | neurotensin + H2O | - |
Bacillus amyloliquefaciens | pyroglutamate + Leu-Tyr-Glu-Asn-Lys-Pro-Ag-Arg-Pro-Tyr-Ile-Leu | - |
? | |
| 3.4.19.3 | thyroliberin-releasing hormone + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.19.3 | trimer | 3 * 24000, SDS-PAGE | Bacillus amyloliquefaciens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.3 | 7.2 | - |
luteinizing-hormone-releasing hormone | - |
Bacillus amyloliquefaciens | |
| 3.4.19.3 | 21 | - |
thyroliberin-releasing hormone | - |
Bacillus amyloliquefaciens | |
| 3.4.19.3 | 26.3 | - |
neurotensin | - |
Bacillus amyloliquefaciens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | 7.5 | - |
- |
Bacillus amyloliquefaciens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.3 | 7 | 8 | stable in presence of 5 mM EDTA and 5 mM 2-mercaptoethanol | Bacillus amyloliquefaciens |
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