EC Number   |
Localization |
GeneOntology No. |
Reference |
|---|
   3.4.22.28 | cytoplasm |
mature 3Cpro and precursor 3CD detected 2-4 hours post-infection of HeLa cells |
GO:0005737 AmiGO QuickGO |
669683 |
| 3.4.22.28 | inclusion body |
recombinant enzyme, expressed in Escherichia coli |
GO:0016234 AmiGO QuickGO |
648003, 648009 |
| 3.4.22.28 | membrane |
- |
GO:0016020 AmiGO QuickGO |
668861 |
| 3.4.22.28 | membrane |
recombinant enzyme |
GO:0016020 AmiGO QuickGO |
648005, 648007 |
| 3.4.22.28 | membrane |
rhinovirus, expressed in Escherichia coli |
GO:0016020 AmiGO QuickGO |
648007 |
| 3.4.22.28 | more |
early in infection, 3Cpro is present as its precursor 3CD, which is found in the nucleus as well as the cytoplasm. Enzyme 2Apro activity (EC 3.4.22.29) is required for 3CD nuclear localization. Nuclear localization of 3CD correlates with 2Apro activity and not 3Cpro activity, which is observed only later in infection. 3Cpro is located in the cytoplasm and the nucleus, whereas 3CD and 3D are localized predominantly in the cytoplasm, implying that 3D lacks nuclear targeting ability and that 3Cpro activity within 3CD is not sufficient to allow the larger protein into the nucleus. The activity of both 2Apro and 3Cpro is required for 3CD entry into the nucleus |
- |
754550 |
| 3.4.22.28 | more |
in the perinuclear membrane |
- |
669683 |
| 3.4.22.28 | more |
localization of the 3C protease in infected and transfected cells is determined using immunofluorescence and confocal microscopy. The DHAV 3C protease is found in the nucleus during infection. In addition, the DHAV 3C protease can enter into the nucleus without cooperation of viral proteins |
- |
755644 |
| 3.4.22.28 | nucleus |
mature 3Cpro and precursor 3CD detected 2-4 hours post-infection of HeLa cells |
GO:0005634 AmiGO QuickGO |
669683 |
| 3.4.22.28 | soluble |
native enzyme: partial |
- |
-, 647997 |
