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EC Number	Purification (Commentary)
1.17.7.3	His6-tagged enzyme. The purified protein is inactive. It is activated after reconstitution of its Fe/S cluster
1.17.7.3	recombinant fusion protein comprising maltose binding protein and IspG protein domains
1.17.7.3	the recombinant MalE/IspG fusion protein (expression level of about 40% with respect to the total soluble cell protein) is purified by affinity chromatography on an amylose resin FF column that is developed with a gradient of 0-10 mM maltose under anaerobic conditions, whereby the enzyme is enriched by a factor of 2.4

Results 1 - 3 of 3

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Release 2023.1 (January 2023)