EC Number |
---|
1.7.2.5 | - |
1.7.2.5 | ammonium sulfate, Resource Q, Resource PHE, Superdex 200 |
1.7.2.5 | DEAE column chromatography and hydroxyapatite column chromatography |
1.7.2.5 | DEAE-Sepharose CL-6B anion-exchange chromatography, Sephacryl S-300 gel filtration, and Mono Q anion-exchange chromatography. Enzyme is solubilized with sucrose monodecanoate from the membranes of Roseobacter denitrificans grown aerobically under light conditions, and purified to electrophoretic homogeneity |
1.7.2.5 | Q-Sepharose anion exchange chromatography |
1.7.2.5 | Q-Sepharose column chromatography and HiTrap chelating Sepharose column chromatography |
1.7.2.5 | recombinant enzyme from Escherichia coli by solubilization with 1% w/v n-dodecyl-beta-D-maltoside, ultracentrifugation, and anion exchange chromatography |
1.7.2.5 | recombinant FlRd |
1.7.2.5 | recombinant His-tagged NorH, wild-type cNOR, and mutant cNOR lacking norH by nickel affinity chromatography from Escherichia coli |
1.7.2.5 | recombinant wild-type and mutant NorC subunits from Escherichia coli strain BL21(DE3) by anion exchange chromatography, gel filtration, and again ion exchange chromatography |