EC Number   |
|---|
   2.7.7.49 | - |
| 2.7.7.49 | cloned from a new HIV-1 group O isolate from Spain and expressed in Escherichia coli |
| 2.7.7.49 | HisTrap column chromatography |
| 2.7.7.49 | improved purification of the 3 enzyme forms: alpha, alpha,beta and beta2 |
| 2.7.7.49 | it is important to purify RT-Ec67 from an Escherichia coli strain defective in DNA polymerase I, because this enzyme can utilize an RNA template to synthesize DNA |
| 2.7.7.49 | Mono Q column chromatography |
| 2.7.7.49 | partial |
| 2.7.7.49 | purification method development, one-step purification 157fold using an affinity column prepared by conjugating an RNase H specific inhibitor with NHS-activated resin, washing with Mn2+, and elution with EDTA, or purification using a C-terminal intein and a biotin tag for avidin affinty chromatography, followed by reductive cleavage of the Mxe intein, overview. Purification of recombinant enzyme from Escherichia coli strain BL21 (DE3). RNase inhibitors have a greater affinity for the RNase H active site in the presence of Mn2+ than in the presence of Mg2+ |
| 2.7.7.49 | recombinant |
| 2.7.7.49 | recombinant enzyme containing the mutations C280S and Q258C |
