EC Number   |
|---|
   3.1.31.1 | - |
| 3.1.31.1 | 10 mM DTT added to expose sulfhydryl groups, and the solution passes through a short column of PD-10 to remove the reducing agent |
| 3.1.31.1 | proline free mutant |
| 3.1.31.1 | recombinant enzyme |
| 3.1.31.1 | recombinant enzyme from Lactococcus lactis cell culture medium by cation exchange chromatography |
| 3.1.31.1 | recombinant fusion protein with Maltose Binding Protein |
| 3.1.31.1 | recombinant GST-tagged enzyme constructs from Escherichia coli strain Rossetta (DE3) by glutathione affinity chromatography, and anion or cation exchange chromatography, followed by dialysis |
| 3.1.31.1 | recombinant His-tagged truncated isozyme Nuc2 lacking the N-terminal membrane anchor by nickel-affinity chromatography |
| 3.1.31.1 | recombinant protein |
| 3.1.31.1 | recombinant protein is purified by anion exchange chromatography on a Q Sepharose Fast Flow column using a linear gradient of NaCl (0-500 mM) in 20 mM Tris-HCl at pH 8.0, 2 mM dithiothreitol and 10% glycerol |
