EC Number |
---|
3.4.21.98 | - |
3.4.21.98 | HiTrap heparin column chromatography, HiTrap SP-HP column chromatography, and Superdex 75 gel filtration |
3.4.21.98 | minimal precursor (residues 9041206 of the hepatitis C virus polyprotein) purified from insoluble inclusion bodies by solubilization in 6 M guanidine hydrochloride and metal chelate resin affinity chromatography |
3.4.21.98 | mutant V36M purified by Ni-resin and gel filtraton |
3.4.21.98 | nickel affinity resin column chromatography and poly-uridine affinity resin column chromatography |
3.4.21.98 | on Ni2+ affinity column |
3.4.21.98 | recombinant GST-tagged enzyme from Escherichia coli TOP cells by glutathione affinity chromatography |
3.4.21.98 | recombinant protein, autolytic conversion of 36 kDa NS2B-NS3 construct into the 30 kDa NS3 enzyme |
3.4.21.98 | recombinant single-chain protease |
3.4.21.98 | single-chain NS3-4A protein purified by gel filtration and on Ni2+-NTA fast flow column, more than 85% pure |