EC Number   |
Storage Stability |
|---|
    1.3.1.12 | -15°C, 0.02% sodium azide, N2-atmosphere, more than 0.1 mg/ml protein |
| 1.3.1.12 | -20°C, 0.1 M N-ethylmorpholine, pH 7.0, 21 mM citrate, 10% v/v glycerol, 1 mM EDTA, 1 mM DTT |
| 1.3.1.12 | -20°C, 0.1 M Tris-HCl buffer, pH 7.5, 1 mM EDTA, 1 mM DTT, 1 mM tyrosine, 70% w/v ammonium sulfate, several weeks |
| 1.3.1.12 | -20°C, 50 mM Cl/N-ethylmorpholine pH 7.2, 1 mM EDTA, 1 mM sodium citrate, 0.02% w/v sodium azide, 50% v/v glycerol, 10 mM DTT, N2-atmosphere, at least 3 months |
| 1.3.1.12 | -20°C, 50 mM phosphate buffer, pH 7.4, at least 0.7 mg/ml protein, 10 months |
| 1.3.1.12 | -80°C, purified recombinant His-tagged wild-type enzyme, complete loss of activity when stored in 0.1 M sodium citrate, pH 7.5, 10% glycerol, 1 mM DTT, or moderate loss in activity when stored in 0.1 M MES, pH 7.5, 0.051 M N-ethylmorpholine, 0.01 M diethanolamine, 1 mM EDTA, 1 mM DTT, 10% glycerol, |
| 1.3.1.12 | -86°C |
| 1.3.1.12 | 0-4°C, 0.1 M Tris-HCl buffer, pH 7.5, 1 mM EDTA, 1 mM DTT, 1 mM tyrosine, 70% w/v ammonium sulfate, for at least 10 days |
| 1.3.1.12 | 4°C, -20°C, or -80°C, 50 mM Tris, 25% glycerol, no significant differences in activity, even after one month of storage |
| 1.3.1.12 | 4°C, 0.1 M N-ethylmorpholine, pH 7.0, 21 mM citrate, 10% v/v glycerol, 1 mM EDTA, 1 mM DTT, up to 4 weeks |
