EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   1.1.1.B20 | more |
2,3-butanediol dehydrogenase (BudC) catalyses the selective asymmetric reductions of prochiral alpha-diketones to the corresponding alpha-hydroxy ketones and diols. BudC is highly active towards structurally diverse diketones in combination with nicotinamide cofactor regeneration systems. Aliphatic diketones, cyclic diketones, and alkyl phenyl diketones are well accepted, whereas their derivatives possessing two bulky groups are not converted. In the reverse reaction vicinal diols are preferred over other substrates with hydroxy/keto groups in non-vicinal positions. Substrate specificity and stereoselectivity, overview. In the reductive reaction diacetyl is the preferred substrate of BudC over acetoin, while only meso-2,3-butanediol oxidation is catalysed by the enzyme under the conditions assessed. No activity with (2S,3S)-butane-2,3-diol and (2R,3R)-butane-2,3-diol. BudC is S-selective for the reduction of diacetyl yielding (S,S)-2,3-butanediol ((S,S)-2,3-BDO), rac-acetoin is reduced to both meso-2,3-BDO and (S,S)-2,3-BDO. Here (R)-acetoin is the preferred substrate and 15% (S)-acetoin remains unconverted after 24 h. Thus, BudC shows a stereo-preference consistent with meso-2,3-butanediol dehydrogenases with respect to acetoin. No activity with R-benzoin, rac-benzoin, benzil, acetone, 2,4-pentanediol, 1,3-butanediol, ethanol, and 2-propanol |
Serratia marcescens CECT 977 |
? |
- |
- |
| 1.1.1.B20 | more |
the enzyme is highly stereospecific, and shows no significant activities towards 2R,3R-2,3-butanediol, 1,4-butanediol, and 2S,3S-2,3-butanediol |
Klebsiella pneumoniae XJ-Li |
? |
- |
? |
| 1.1.1.B20 | more |
production of S,S- and/or R,R- and meso-2,3-BDO by Paenibacillus brasilensis strain PB24 grown in the modified YEPD medium, pH 6.3, 32°C, up to 72 h |
Paenibacillus brasilensis PB24 |
? |
- |
- |
| 1.1.1.B20 | more |
stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A |
Bacillus licheniformis 10-1-A |
? |
- |
? |
| 1.1.1.B20 | more |
the enzyme is also active with diacetyl and NADH |
Klebsiella aerogenes KCTC 2190 |
? |
- |
? |
| 1.1.1.B20 | more |
substrate promiscuity of the SmBdh enzyme, SmBdh has been reported to be able to reduce both (3R)- and (3S)-acetoin to 2,3-BDO, although (3R)-acetoin is more readily converted than (3S)-acetoin. On the other hand, SmBdh is able to oxidize meso-2,3-BDO and (2S,3S)-BDO, while oxidation of (2R,3R)-BDO is not detectable. Moreover, its activity towards (2S,3S)-BDO is only 11% of that towards meso-2,3-BDO. The enzyme is classified as an S-acting Bdh based on production of meso-2,3-BDO and (2S,3S)-BDO from a racemic mixture of acetoin |
Serratia marcescens 9C |
? |
- |
- |
| 1.1.1.B20 | more |
enzyme BtBDH is active with meso-2,3-butanediol and (2R,3R)-2,3-butanediol, whereas no activity is observed with (2S,3S)-2,3-butanediol. BtBDH shows similar oxidative activity toward meso-2,3-butanediol and (2R,3R)-2,3-butanediol, and it exhibits a 3fold higher reduction activity toward acetoin compared to diacetyl |
Bacillus thuringiensis serovar kurstaki ACCC 10066 |
? |
- |
- |
| 1.1.1.B20 | propane-1,2-diol + NAD+ |
- |
Pseudomonas aeruginosa |
? + NADH + H+ |
- |
? |
| 1.1.1.B20 | rac acetoin + NADH + H+ |
- |
Pseudomonas aeruginosa |
(2R,3R)-butane-2,3-diol + NAD+ |
- |
? |
| 1.1.1.B20 | rac acetoin + NADH + H+ |
- |
Pseudomonas aeruginosa |
(2R,3S)-butane-2,3-diol + NAD+ |
- |
? |
